Comparison of Listeria monocytogenes virulence in a mouse model

Journal of Food Protection
Kazue TakeuchiM A Smith

Abstract

Listeriosis results from exposure to the foodborne pathogen Listeria monocytogenes. Although many different strains of L. monocytogenes are isolated from food, no definitive tests currently predict which isolates are most virulent. The objectives of this study were to address two major data gaps for risk assessors, variability among L. monocytogenes strains in pathogenicity and virulence. Strains used in our monkey clinical trial or additional food isolates were evaluated for their virulence and infectivity in mice. All strains were equally pathogenic to immunocompromised mice, causing deaths to 50% of the population 3 days after exposure to doses ranging from 2 to 3 log CFU. Doses resulting in 50% deaths on the fifth day after administration were 1 to 2 log lower than those on the third day, indicating that the full course of pathogenicity exceeds the 3-day endpoint in immunocompromised mice. Three strains were chosen for further testing for their virulence and infectivity in liver and spleen in normal (immunocompetent) mice. Virulence was not significantly different (P > 0.05) among the three strains, all resulting in deaths to 50% of mice at 5 to 7 log CFU by 5 days after administration. All strains were equally infective in...Continue Reading

References

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Feb 22, 2003·Infection and Immunity·Mary Alice SmithMichael P Doyle
Dec 16, 2003·Journal of Food Protection·Kazue TakeuchiMichael P Doyle
Dec 1, 1991·Journal of Food Protection·Alain MenudierJean-Albert Nicolas

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Citations

Dec 17, 2008·FEMS Microbiology Letters·Bent B RoldgaardTine Rask Licht
Nov 12, 2009·Journal of Food Protection·Arena N RichardsonMary Alice Smith
Aug 31, 2007·International Journal of Food Microbiology·Dongyou LiuFrank W Austin

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