Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Stem Cell Reports
Johannes RieglerJoseph C Wu

Abstract

The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm(3). A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm(3) and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.

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Citations

Dec 17, 2016·Nature Reviews. Drug Discovery·Yanhong ShiShinya Yamanaka
Sep 7, 2017·Nature Nanotechnology·Morteza MahmoudiOmid C Farokhzad
Aug 2, 2018·Cellular and Molecular Life Sciences : CMLS·Andreas Brown, Hartmut Geiger
Feb 12, 2017·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Davis Vo, Patricia K Nguyen

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Methods Mentioned

BETA
genetic modification
PCR
ELISAs
ELISA

Software Mentioned

R

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