Comparison of Mechanisms of Endothelial Cell Protections Between High-Density Lipoprotein and Apolipoprotein A-I Mimetic Peptide

Frontiers in Pharmacology
Wenqi XuDonghai Lin

Abstract

Apolipoprotein A-I (apoA-I) mimetic peptide, D-4F, exhibits anti-atherogenic effects similar to high-density lipoprotein (HDL). However, it remains elusive whether D-4F and HDL share similar molecular mechanisms underlying anti-atherogenic effects and endothelial cell protections. We here compared the metabolic changes in endothelial cells induced by D-4F and HDL against oxidized low-density lipoprotein (ox-LDL), which may be of benefit to understanding the protective mechanisms of HDL and D-4F. Functional assays, including wound healing, transwell migration, and tube formation, were used to evaluate the pro-angiogenic effects of HDL and D-4F. NMR-based metabolomic analysis was employed to explore the protective mechanisms underlying HDL and D-4F. Partial least-squares discriminant analysis (PLS-DA) was performed to assess metabolic profiles, and orthogonal PLS-DA (OPLS-DA) was carried out to identify characteristic metabolites. Moreover, significantly altered metabolic pathways were also analyzed. We found that ox-LDL impaired the migration and tube formation of endothelial cells. Metabolomic analysis showed that ox-LDL triggered oxidative stress, impaired glycolysis, and enhanced glycerophospholipid metabolism. Both HDL and D...Continue Reading

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Citations

Apr 17, 2020·Frontiers in Cardiovascular Medicine·Anne Jomard, Elena Osto
Apr 4, 2021·Molecular and Cellular Biochemistry·Ampadu O JacksonShiyin Long
Aug 24, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zhe-Bin ChengChi Zhang

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Methods Mentioned

BETA
metabolomic assay
nuclear magnetic resonance
electrophoresis
light microscopy
NMR
1
H
PCA
metabolomic profiling

Software Mentioned

DA
Chenomx NMR Suite
MetaboAnalyst
MatLab
SPSS
MestReNova
PLS
GraphPad Prism
ImageJ
SIMCA

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