PMID: 8938862Nov 1, 1996Paper

Comparison of media and conditions of incubation for the quantitative culture of Malassezia pachydermatis from canine skin

Research in Veterinary Science
R Bond, D H Lloyd

Abstract

Fifty-five swab-wash specimens from dogs were cultured at 32 degrees C on Sabouraud's dextrose agar either with or without 1 per cent Tween 80. Ushijima's medium A and modified Dixon's and Leeming's media. The counts of Malassezia pachydermatis were not significantly different after three or seven days of incubation. Colony counts on contact plates were significantly greater after incubation for seven days on Sabouraud's dextrose (P < 0.001) and modified Dixon's agars (P < 0.05) than after three days at 26 degrees C, but not at 32 degrees C. Counts on Sabouraud's or modified Dixon's agars after incubation at 32 degrees C and 37 degrees C were not significantly different. When compared with aerobic culture, an atmosphere containing 5 to 10 per cent carbon dioxide significantly (P < 0.01) increased the frequency of isolation and colony counts on Sabouraud's dextrose agar but not on modified Dixon's agar.

Citations

Jan 5, 2011·Molecular and Cellular Probes·Claudia CafarchiaDomenico Otranto
Oct 24, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J GuillotR Chermette
Nov 15, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J L BlancoM E García
Oct 16, 1999·Medical Mycology·J Guillot, R Bond
May 21, 2020·Frontiers in Cellular and Infection Microbiology·Maritza TorresAdriana Marcela Celis Ramírez

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