Comparison of Media for Recovery of Verticillium dahliae from Soil

Plant Disease
Z KabirKrishna V Subbarao

Abstract

Polygalacturonic acid (PGA) is an important constituent of Sorensen's NP-10 medium (NP-10) for estimating the population density of Verticillium dahliae in soil. Different types of PGA are available, but not all of them favor the growth of V. dahliae. Unavailability of PGA sodium salt from orange (P-1879) has created an unprecedented problem for the quantification of microscle-rotia (MS) of V. dahliae in soil. The PGA from orange (P-3889) that is now available does not support the growth of V. dahliae. Therefore, experiments were conducted to optimize the use of NP-10 prepared with P-3889 and various concentrations of NaOH. NP-10 with P-3889 amended with eight concentrations of NaOH were compared with NP-10 prepared from PGA sodium salt from orange (P-1879, now discontinued) and citrus (P-3850) along with cellophane and Na-pectate media for recovery of MS from soil and growth of V. dahliae on the media. Seven soils were assayed for MS, and eight isolates of V. dahliae were evaluated for growth and production of MS. Concentrations of NaOH >0.035N and <0.02N in NP-10 with P-3889 reduced mycelial growth, microsclerotial production, and recovery of MS from soils. Similarly, NP-10 with P-3850 alone, cellophane, and Na-pectate media ...Continue Reading

References

Oct 6, 1998·Applied and Environmental Microbiology·A J TermorshuizenL Tsror
Oct 24, 2008·Phytopathology·R G Bhat, K V Subbarao

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Citations

Jan 8, 2019·Phytopathology·David Linnard Wheeler, Dennis Allen Johnson
Jan 2, 2020·Pathogens·Mandela Elorm AddrahJun Zhao
Feb 14, 2019·PloS One·David Linnard WheelerDennis Allen Johnson
Jul 14, 2017·Phytopathology·Christine L CarrollKrishna V Subbarao
Nov 10, 2011·Phytopathology·Guillaume J BilodeauFrank N Martin
Oct 1, 2010·Plant Disease·Matteo CirulliRafael M Jiménez-Díaz
Aug 1, 2007·Plant Disease·K V SubbaraoS T Koike
Aug 1, 2016·Plant Disease·Rumakanta SapkotaMogens Nicolaisen
Jul 26, 2017·Archives of Microbiology·Georgios TzelepisChristina Dixelius
May 14, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ryan J HayesKrishna V Subbarao
Feb 2, 2016·Phytopathology·David Linnard Wheeler, Dennis A Johnson
Jul 1, 2015·Plant Disease·Daniel Jiménez-FernándezRafael M Jiménez-Díaz

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