Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide) copolymer film and by trypsinization

Biomedical Materials
Lei YangWenhua Guo

Abstract

Temperature-responsive platforms containing poly(N-isopropylacrylamide) (PNIPAAm) have been developed as an effective substitute for enzymatic treatment to recover adherent cells, but it remains unclear whether this alternative harvesting method tends to support stem cells preserving them being primitive. This study mainly investigated the biological properties of mesenchymal stem cells derived from rat bone marrow and human adipose tissue (BM-MSCs and AT-MSCs) after being cultured on PNIPAAm copolymer films and recovered by temperature drop, and compared the cells harvested from glass coverslips with trypsinization as controls. The experimental results demonstrated that after three serial passages, the released MSCs from thermal liftoff showed no significant differences in cell morphology, immunophenotype and osteogenesis for BM-MSCs or adipogenesis for AT-MSCs, but had higher viability, stronger proliferation and higher adipogenic differentiation for BM-MSCs or higher osteogenic differentiation for AT-MSCs compared with the trypsinization group. Besides, more proteins remained around or within the cell membranes upon temperature drop. It is concluded that cell detachment with more extracellular matrix proteins facilitates the...Continue Reading

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Citations

Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Kedong SongTianqing Liu
Oct 28, 2016·Journal of Biomedical Materials Research. Part a·Thafar AlmelaKeyvan Moharamzadeh
Aug 25, 2015·Macromolecular Rapid Communications·Xingliang FanYury A Rochev
Dec 30, 2016·Future Science OA·Vanessa PennaSilvio E Duailibi
Aug 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Audrey RoyJean-Luc Six
Apr 11, 2016·ACS Biomaterials Science & Engineering·Yinan MaKeiji Numata

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