Comparison of Metal-Ammine Compounds Binding to DNA and Heparin. Glycans as Ligands in Bioinorganic Chemistry

Inorganic Chemistry
Samantha J KatnerNicholas P Farrell

Abstract

We present spectroscopic and biophysical approaches to examine the affinity of metal-ammine coordination complexes for heparin as a model for heparan sulfate (HS). Similar to nucleic acids, the highly anionic nature of heparin means it is associated in vivo with physiologically relevant cations, and this work extends their bioinorganic chemistry to substitution-inert metal-ammine compounds (M). Both indirect and direct assays were developed. M compounds are competitive inhibitors of methylene blue (MB)-heparin binding, and the change in the absorbance of the dye in the presence or absence of heparin can be used as an indirect reporter of M-heparin affinity. A second indirect assay uses the change in fluorescence of TAMRA-R9, a nonaarginine linked to a fluorescent TAMRA moiety, as a reporter for M-heparin binding. Direct assays are surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC). The Kd values for TriplatinNC-heparin varied to some extent depending on the technique from 33.1 ± 2 nM (ITC) to 66.4 ± 1.3 nM (MB absorbance assay) and 340 ± 30 nM (SPR). The differences are explained by the nature of the technique and the use of heparin of differing molecular weight. Indirect probes using the displacement of...Continue Reading

References

Dec 1, 1989·Journal of Inorganic Biochemistry·E Tselepi-Kalouli, N Katsaros
Dec 17, 1997·Journal of Colloid and Interface Science·K Gaus, E A H Hall
Aug 31, 1999·Biophysical Journal·H Deng, V A Bloomfield
Jan 12, 2002·Current Opinion in Structural Biology·S Leavitt, E Freire
Nov 9, 2002·Analytical Biochemistry·Ronald I W OsmondDeirdre R Coombe
Dec 20, 2002·Angewandte Chemie·Ishan Capila, Robert J Linhardt
Mar 3, 2004·Biochemistry·Stephen M Fuchs, Ronald T Raines
Apr 8, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Li ZhangKe'an Li
Feb 14, 2006·Oncogene·L BarazI Vlodavsky
Aug 3, 2006·The Journal of Biological Chemistry·Chenghua ShaoBarbara A Seaton
Dec 18, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D BharanidharanN Gautham
Apr 10, 2009·Current Medicinal Chemistry·Huiqi Xie, Y James Kang
Jan 30, 2010·International Journal of Molecular Sciences·Alan Brown
Mar 12, 2010·PLoS Computational Biology·Sean R McGuffee, Adrian H Elcock
Jun 11, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Luca Domenico D'AndreaCarlo Pedone
Oct 29, 2010·Cell Biochemistry and Biophysics·Ivan StevicAnthony K C Chan
Dec 2, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hunghao ChuYadong Wang
Nov 26, 2013·Chemistry & Biology·Jason E Hudak, Carolyn R Bertozzi
Jan 21, 2014·Chemistry & Biology·Richard D Cummings, J Michael Pierce
Jan 28, 2014·Chemical Communications : Chem Comm·John B MangrumNicholas P Farrell
Feb 26, 2014·Biochemical Pharmacology·Claudio PisanoFranco Zunino
Mar 13, 2014·Annual Review of Biochemistry·Ding Xu, Jeffrey D Esko
Apr 14, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paola ChiodelliMarco Rusnati
Aug 21, 2015·Journal of the Royal Society, Interface·Maria C Z MeneghettiMarcelo A Lima
Jan 29, 2016·International Journal of Molecular Sciences·Xing DuShu-Qun Liu

❮ Previous
Next ❯

Citations

Aug 6, 2019·The FEBS Journal·Mariana P StellingNicholas P Farrell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.