Comparison of methods for counting cells in the mouse glomerulus

Nephron. Experimental Nephrology
J M BasgenJ R Nyengaard

Abstract

Researchers have long been interested in counting the number of cells within the glomerulus. Investigators using different techniques have yielded conflicting results. The most direct method is to count the cells in serial sections from the entire glomerulus. This Exhaustive Count method is not usually practical. The disector/fractionator method counts the number of cells in a fraction of sections. The total is estimated from the product of the cell count and the reciprocal of the fraction. The Weibel-Gomez method determines the density of cells per glomerulus, then multiplies this density by glomerular volume to obtain cell number. In this study, we compared the disector/fractionator and Weibel-Gomez methods to identify a practical alternative for the time-consuming Exhaustive Count method. Glomeruli from a normal mouse kidney were completely sectioned and images obtained. Appropriate images were used to count glomerular cell number using each method. The Exhaustive Count method yielded 213 +/- 22 (mean +/- SD) cells/glomerulus vs. the disector/fractionator average of 211 +/- 29 cells/glomerulus (p = 0.82). The Weibel-Gomez method average of 235 +/- 26 cells/glomerulus was statistically different from the Exhaustive Count meth...Continue Reading

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Citations

Jun 10, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Dong-Sub JungShin-Wook Kang
Oct 11, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Victor G PuellesJames A Armitage
Jan 5, 2011·American Journal of Nephrology·Xiao Yan Bai, John M Basgen
Jun 17, 2011·American Journal of Nephrology·Susanne B NicholasSatyesh Sinha
Jul 18, 2008·Journal of the American Society of Nephrology : JASN·Shirong ZhengPaul N Epstein
Sep 12, 2012·Journal of Neuropathology and Experimental Neurology·Robert M GrumblesChristine K Thomas
Mar 16, 2016·Journal of the American Society of Nephrology : JASN·Victor G PuellesJohn F Bertram
Dec 19, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jennifer M TeikenEdward C Carlson
Sep 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cynthia A BatchelderAlice F Tarantal
Oct 27, 2010·Muscle & Nerve·Gizelda T B CasellaChristine K Thomas
Jun 30, 2007·Journal of the American Society of Nephrology : JASN·Jin-Ju KimShin-Wook Kang
Jul 9, 2013·Journal of the American Society of Nephrology : JASN·Kevin V LemleyKathryn White
Dec 21, 2013·Journal of the American Society of Nephrology : JASN·Madhusudan VenkatareddyRoger C Wiggins
Apr 17, 2009·American Journal of Physiology. Renal Physiology·Jin Ji LiShin-Wook Kang
Apr 30, 2009·Diabetes·Sarah A RoxburghDerek P Brazil
Oct 24, 2018·BMC Nephrology·Paschal RuggajoBjørn Egil Vikse
Jun 1, 2017·Cell and Tissue Research·Victor G PuellesMarcus J Moeller
Nov 5, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Edward C CarlsonYi Tan

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