PMID: 11329666May 2, 2001Paper

Comparison of methods for determination of microbial biomass in wastewater

Water Research
J VollertsenPer Halkjaer Nielsen

Abstract

Microbial biomass in wastewater was determined by methods used in environmental microbiology and by a method used in wastewater engineering based on a conceptual model simulating fundamental microbial processes in wastewater from measured oxygen uptake rates. The methods originating from environmental microbiology are based on staining and counting of cells for the determination of total cell biomass (acridine orange and DAPI), physiological state of cells (LIVE/DEAD BacLight) and activity of cells (reduction of the redox dye CTC and microautoradiography). Depending on the staining method applied, cell biomasses yielded 15-86% of the biomass defined by the model, and good correlations between cell biomass and model biomass were found. Cell biomass, oxygen uptake and acetate uptake were measured in wastewater, where acetate was added. Substrate uptake rates were found not to be proportional to the increases in cell biomass, suggesting that only a small fraction of the cell biomass was responsible for the main part of the substrate uptake. Despite the differences found between cell biomass and model biomass, it was recommended to use the conceptual model as an engineering tool for simulation of microbial processes and wastewater ...Continue Reading

References

Dec 15, 1992·FEMS Microbiology Letters·D W Tempest, O M Neijssel
Jan 1, 1984·Annual Review of Microbiology·D W Tempest, O M Neijssel
Jun 19, 2003·The Journal of Trauma·David E Clark
Jan 20, 1989·Biotechnology and Bioengineering·M H Turakhia, W G Characklis

❮ Previous
Next ❯

Citations

Nov 11, 2011·Applied Microbiology and Biotechnology·Xiangjuan YuanHong Liang
Sep 8, 2010·Journal of Industrial Microbiology & Biotechnology·Ramazan Ali Dianati Tilaki
Mar 26, 2008·Chemical Society Reviews·Sébastien JossetValérie Keller
Mar 19, 2003·Environmental Technology·N Muhammad, A Morris Hooke
Aug 25, 2004·Journal of Microbiological Methods·Nicolas RousselonJean-Jacques Godon
Jul 1, 2016·Water Research·E ScholesP Brook-Carter
Oct 30, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Steffen EngelbrechtMarion Martienssen
Aug 26, 2006·Tropical Medicine & International Health : TM & IH·Thomas ClasenShashikala Menon
Aug 12, 2014·Bulletin of Environmental Contamination and Toxicology·Usha Pandey, Jitendra Pandey
May 24, 2013·Bulletin of Environmental Contamination and Toxicology·Jitendra PandeyRachna Singh
Nov 25, 2014·Applied Microbiology and Biotechnology·Jitendra KeshriMaggy N B Momba
Mar 17, 2016·Applied Microbiology and Biotechnology·I KamikaM Tekere
Sep 6, 2011·Applied Biochemistry and Biotechnology·Fathollah Gholami-BorujeniMahmood Alimohammadi
Jun 11, 2014·Food Additives & Contaminants. Part B, Surveillance·Ilunga KamikaDavid R Katerere
Jul 30, 2017·International Journal of Environmental Research and Public Health·Avhashoni D NefaleMaggy Nb Momba
Jan 19, 2018·FEMS Microbiology Ecology·Pablo AguilarRuben Sommaruga
Mar 16, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Behrooz RazbanDena W McMartin
Mar 1, 2020·Applied and Environmental Microbiology·Jannie Munk KristensenPer Halkjær Nielsen
May 19, 2017·Bulletin of Environmental Contamination and Toxicology·Amita Yadav, Jitendra Pandey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.