PMID: 9421715Jan 9, 1998Paper

Comparison of methods for recovery of Ascaris suum larvae from tissues of mice

International Journal for Parasitology
H C SlotvedP Nansen

Abstract

Experiments were undertaken to compare procedures for isolating Ascaris suum from mice. A standardised intragastric procedure for inoculation of A. suum eggs, which had a very low interdose variation (S.D. = 6.2% of mean inoculation dose), was developed. There was no significant difference in the number of larvae recovered from the large intestine 4 h post inoculation (p.i.) by using either Baermannisation of washed intestinal wall or submucosal scrapings. In contrast, a significantly higher recovery of larvae was achieved by incubating the intestines vertically in a cylindrical saline-filled funnel. An agar-gel method was used to recover larvae from mucosal scrapings; however, the number of larvae recovered was the lowest of the methods tried. It was found that a significant increase in larval recovery from the liver at 24 h p.i. resulted when the liver was pressed through a garlic press, rather than by using homogenisation. For lung recovery, the highest recovery of larvae (at day 8 p.i.) was obtained by disintegration of tissue with a pair of scissors and incorporating the tissue into agar-gel. The methods presented in this study may be useful for investigation of the histology, morphology and molecular biology of the early ...Continue Reading

Citations

Oct 16, 2004·Veterinary Parasitology·I E SommerfeltR De Torres
Mar 28, 2012·Experimental Parasitology·Weidong PengNingyan Hu
Dec 3, 2020·PLoS Neglected Tropical Diseases·Tao WangRobin B Gasser

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