Abstract
Experiments were undertaken to compare procedures for isolating Ascaris suum from mice. A standardised intragastric procedure for inoculation of A. suum eggs, which had a very low interdose variation (S.D. = 6.2% of mean inoculation dose), was developed. There was no significant difference in the number of larvae recovered from the large intestine 4 h post inoculation (p.i.) by using either Baermannisation of washed intestinal wall or submucosal scrapings. In contrast, a significantly higher recovery of larvae was achieved by incubating the intestines vertically in a cylindrical saline-filled funnel. An agar-gel method was used to recover larvae from mucosal scrapings; however, the number of larvae recovered was the lowest of the methods tried. It was found that a significant increase in larval recovery from the liver at 24 h p.i. resulted when the liver was pressed through a garlic press, rather than by using homogenisation. For lung recovery, the highest recovery of larvae (at day 8 p.i.) was obtained by disintegration of tissue with a pair of scissors and incorporating the tissue into agar-gel. The methods presented in this study may be useful for investigation of the histology, morphology and molecular biology of the early ...Continue Reading
References
Feb 1, 1992·Veterinary Parasitology·L EriksenJ Urban
May 1, 1968·Parasitology·D C Jenkins
Jan 1, 1968·Zeitschrift Für Parasitenkunde·M Buske, H Engelbrecht
Jun 1, 1996·Veterinary Parasitology·H C SlotvedP Nansen
Jun 11, 2002·The Journal of Parasitology·J BoesN Agergaard
Apr 1, 1964·Zeitschrift Für Parasitenkunde·D K BHOWMICK
Citations
Aug 24, 2012·Parasitology Research·Tania FerdushyNiels C Kyvsgaard
Jun 24, 1998·Veterinary Parasitology·J BoesP Nansen
Oct 8, 2005·Parasitology·R LewisC V Holland
Jul 13, 2006·Parasitology·W PengR B Gasser
Mar 27, 2007·Parasitology·R LewisC V Holland
Feb 22, 2012·DNA and Cell Biology·Guangzhi HeGuangyou Yang
Nov 14, 2001·Infection and Immunity·N TsujiT Isobe
Aug 23, 2003·Infection and Immunity·Naotoshi TsujiYasunobu Matsumoto
Apr 20, 2013·International Journal for Parasitology·L HonglinM Zhouxu
May 12, 2009·Vaccine·Guangzhi HeLi Liu
Oct 22, 2008·Vaccine·Tao WangLi Liu
Oct 16, 2004·Veterinary Parasitology·I E SommerfeltR De Torres
Oct 8, 2004·Parasitology International·Chia-Kwung Fan, Kua-Eyre Su
Apr 3, 2012·Vaccine·Ning ChenXing-Quan Zhu
Mar 28, 2012·Experimental Parasitology·Weidong PengNingyan Hu
May 9, 2014·PloS One·Andrew R WilliamsStig M Thamsborg
Jul 7, 2005·International Journal for Parasitology·M Khyrul IslamNaotoshi Tsuji
Sep 24, 2004·Veterinary Parasitology·Pia WebsterChristian M O Kapel
Mar 27, 2007·Journal of Helminthology·S ChoN Akao
Dec 3, 2020·PLoS Neglected Tropical Diseases·Tao WangRobin B Gasser
Feb 23, 2021·Parasitology·C V Holland