Comparison of methods for the isolation of mycobacteria from water treatment plant sludge

Antonie van Leeuwenhoek
Jitka MakovcovaIva Slana

Abstract

Nontuberculous mycobacteria (NTM) are ubiquitous organisms in all natural ecosystems, including water environments. Several of these species are potential pathogens which affect human health. NTM most commonly cause pulmonary, skin or soft tissue infections. Primary sludge obtained from the water treatment plants of four drinking water reservoirs were subjected to analysis for mycobacteria. Five decontamination methods (5% oxalic acid, modified Petroff, HCl-NaOH, N-acetyl-L-cysteine-sodium hydroxide and 0.05% cetylpyridinium chloride), three growth media (Herrold's egg yolk medium with and without the antibiotic cocktail PANTA and Löwenstein-Jensen medium with sodium pyruvate) and three incubation temperatures (25, 30 and 37 °C) for isolation of mycobacteria were compared in the analysis of 18 sludge samples. To evaluate examined methods, the overall positive, negative, and contamination rate, and these rates in respect to localities are taken into account. Statistical analysis demonstrated that the best combination for the recovery of mycobacteria with the minimum number of contaminating microorganisms is 5% oxalic acid decontamination cultured on Herrold's egg yolk medium with the antibiotic cocktail PANTA at 25 °C. The least...Continue Reading

References

Oct 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D L WhippleJ L Jarnagin
Jan 1, 1988·Microbiology and Immunology·S IchiyamaM Tsukamura
Mar 1, 1988·Journal of General Microbiology·F PortaelsM P Sylla
Jan 1, 1980·Applied and Environmental Microbiology·D J DudleyB P Sagik
Jan 1, 1981·Canadian Journal of Microbiology·J G Songer
Aug 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R Schulze-RöbbeckeG Wahl
Jun 1, 1995·The Journal of Applied Bacteriology·E Livanainen
Apr 1, 1993·International Journal of Systematic Bacteriology·J KazdaZ S Tarnok
May 1, 1995·Clinics in Dermatology·S J Gluckman
Aug 8, 1996·The New England Journal of Medicine·C R Horsburgh
Apr 1, 1996·Clinical Microbiology Reviews·J O Falkinham
Aug 1, 1997·Applied and Environmental Microbiology·I T MiettinenP J Martikainen
May 29, 1999·Applied and Environmental Microbiology·T C CovertG N Stelma
Sep 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P M ArnowJ L Bova
Sep 14, 2001·Letters in Applied Microbiology·L B WhanM T Rowe
Oct 5, 2002·Clinical Microbiology Reviews·Barbara A Brown-Elliott, Richard J Wallace
Oct 10, 2002·Clinics in Chest Medicine·Joseph O Falkinham
Oct 31, 2002·Applied and Environmental Microbiology·Corinne Le DantecVéronique Vincent
Nov 13, 2003·BMC Infectious Diseases·Dag HarmsenElvira Richter
Jan 17, 2004·Clinical Microbiology Reviews·Todd P PrimmJoseph O Falkinham
Oct 13, 2005·FEMS Microbiology Reviews·Mario J M VaerewijckFrançoise Portaels
Mar 1, 1994·Applied and Environmental Microbiology·T KamalaR Prabhakar
Feb 10, 2006·Diagnostic Microbiology and Infectious Disease·Toyomitsu SawaiShigeru Kohno
Feb 1, 1997·Applied and Environmental Microbiology·M NeumannK Behringer
Jun 2, 2006·International Journal of Systematic and Evolutionary Microbiology·Joann L CloudGail L Woods
Sep 8, 2006·Applied and Environmental Microbiology·Elizabeth D HilbornGerard N Stelma
Feb 6, 2007·American Journal of Respiratory and Critical Care Medicine·David E GriffithUNKNOWN Infectious Disease Society of America
Nov 6, 2007·International Journal of Systematic and Evolutionary Microbiology·Christopher M WhippsMichael L Kent
Jun 9, 2009·International Journal of Systematic and Evolutionary Microbiology·Enrico TortoliJakko van Ingen
Jul 22, 2009·International Journal of Systematic and Evolutionary Microbiology·J van IngenD van Soolingen
Oct 30, 2009·Journal of Clinical Microbiology·Miriam GrechRachel Thomson
Apr 7, 2010·Applied and Environmental Microbiology·Nicolas RadomskiFrançoise S Lucas
Apr 21, 2010·International Journal of Hygiene and Environmental Health·Daniele CorsaroGilbert Greub
Sep 14, 2010·International Journal of Systematic and Evolutionary Microbiology·Naima SahraouiPeter M Keller
Feb 22, 2012·International Journal of Systematic and Evolutionary Microbiology·Geoffrey D HanniganJohn L Dahl
Mar 22, 2013·World Journal of Microbiology & Biotechnology·Dan-Ning ZengZhe-Xue Quan

❮ Previous
Next ❯

Citations

Dec 2, 2015·Experimental and Therapeutic Medicine·Ioannis K NeonakisZoe Gitti
Jul 25, 2015·Genome Announcements·Alexander L GreningerSteve Miller
Aug 11, 2017·The American Journal of Surgical Pathology·Isaac H SolomonDanny A Milner
Sep 15, 2018·Frontiers in Microbiology·Jennifer R HondaEdward D Chan
Aug 30, 2019·The Journal of Veterinary Medical Science·Gisele BacanelliNewton V Verbisck
May 22, 2021·Microbial Genomics·Rudielle de Arruda RodriguesAndries J van Tonder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.