PMID: 2509112Oct 1, 1989Paper

Comparison of methods for the measurement of apoproteins A1 and B in serum

Clinical Biochemistry
M T Meuffels, J T Hindmarsh

Abstract

We evaluated four currently available commercial methods for measuring the concentration of apoproteins A1 and B in human serum (a radioimmunoassay, an immunonephelometric assay, and two electroimmunoassays). We found that the immunonephelometric assay provided the best between-run precision, whereas the two electroimmunoassays gave the poorest. Only the immunoephelometric assay achieved a between-run coefficient of variation of less than 5%, which we believe is a desirable target for this parameter. Comparison with other laboratories was assessed using a reference serum obtained from the Centers for Disease Control, Atlanta, Georgia, USA; we are currently using this as a reference serum employing a range of +/- 15% of its assigned values as acceptable. Linearity of the immunonephelometric assay was satisfactory throughout the ranges of 0.5-2.0 g/L for apoprotein A1, and 0.3-1.8 g/L for apoprotein B.

References

Nov 1, 1987·Atherosclerosis·P PuchoisJ C Fruchart
May 1, 1986·Mayo Clinic Proceedings·B A KottkeS J Mao
Aug 18, 1983·The New England Journal of Medicine·J J MaciejkoS J Mao
Apr 1, 1982·Atherosclerosis·G De BackerJ P Deslypere
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A SnidermanP O Kwiterovich

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