PMID: 11312828Apr 21, 2001Paper

Comparison of methylation procedures for conjugated linoleic acid and artifact formation by commercial (trimethylsilyl) diazomethane

Journal of Agricultural and Food Chemistry
Y ParkM W Pariza

Abstract

Four different methods for methylating conjugated linoleic acid (CLA) were compared. The HCl/MeOH and BF(3)/MeOH methods were tested under different time and temperature combinations. Increasing temperature and/or incubation time for either method decreased the cis-9,trans-11 and trans-10,cis-12 isomers, but trans-9,trans-11/trans-10,trans-12 isomers and artifacts (allylic methoxide) were increased. In addition, the triacylglyceride form of CLA was tested using the above methods and NaOMe at various temperatures for 20 min. The NaOMe did not generate methoxy artifacts. However, there were impurities in GC after methylation with NaOMe as well as with BF(3)/MeOH. The (trimethylsilyl)diazomethane method, which is a mild and easy alternative, was tested. Free forms of fatty acids were easily, but not completely, methylated by this method. Also, the method generated artifacts (trimethylsilyl CLA esters) and impurities (trimethylsilyl) that would interfere with short-chain fatty-acid analysis by GC.

References

Jan 13, 2000·Proceedings of the Society for Experimental Biology and Medicine·M W ParizaM E Cook

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Citations

Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A BuccioniM Mele
Aug 8, 2007·Chemistry and Physics of Lipids·Jingnan ChenZhen-Yu Chen
Apr 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna TopolewskaPiotr Stepnowski
Apr 22, 2004·European Journal of Mass Spectrometry·John M Halket, Vladimir V Zaikin
Aug 11, 2007·Angewandte Chemie·Erik KühnelJennifer L Slaughter
Jun 27, 2015·Journal of Animal Science·M LanzaG Luciano
Nov 29, 2013·The Journal of Nutrition·Shane M HuebnerMark E Cook
Oct 16, 2007·Analytica Chimica Acta·Carolina Moltó-PuigmartíM Carmen López-Sabater

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