Comparison of MITF and Melan-A Immunohistochemistry During Mohs Surgery for Lentigo Maligna-Type Melanoma In Situ and Lentigo Maligna Melanoma

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]
Kevin N ChristensenChristopher J Arpey

Abstract

Mohs micrographic surgery (MMS) with frozen section immunohistochemistry is a treatment option for malignant melanoma in situ (MMIS) and lentigo maligna melanoma (LMM). Melan-A is a cytoplasmic melanocyte immunostain useful on frozen sections but may lack specificity. Microphthalmia transcription factor (MITF) is a more specific nuclear melanocyte immunostain less frequently used in MMS. To quantify melanocyte density in chronic sun-damaged skin (CSDS), negative margin, and tumor from patients undergoing MMS for MMIS and LMM using MITF and melan-A. Sixteen patients with MMIS or LMM had frozen sections from CSDS, negative margin, and 12 tumor samples, stained with MITF and melan-A. Melanocyte counts were performed. Chronic sun-damaged skin mean melanocyte count (MMC) for MITF and melan-A was 9.8 and 13.7, respectively, (p < .001). Negative margin MMC for MITF and melan-A was 8.84 and 14.06, respectively, (p < .001). Tumor MMC for MITF and melan-A was 63.5 and 62.4, respectively. Although both MITF and melan-A facilitate the identification of tumor during MMS for MMIS and LMM, the apparent melanocyte density on tumor-free CSDS appears higher with melan-A than MITF. Microphthalmia transcription factor provides a crisp outline of m...Continue Reading

References

Aug 1, 1997·Journal of the American Academy of Dermatology·J A ZitelliB H Hanusa
Jul 2, 1998·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·L M CohenR H Zax
Aug 15, 1998·Journal of the American Academy of Dermatology·S M AckerJ C Maize
Mar 26, 2003·The American Journal of Dermatopathology·John C MaizePhilip E LeBoit
Sep 15, 2004·The American Journal of Dermatopathology·Laila El Shabrawi-CaelenLorenzo Cerroni
Jan 1, 2005·Journal of the American Academy of Dermatology·Gregory M BriccaJohn A Zitelli
Sep 18, 2007·Journal of the American Academy of Dermatology·Hobart W WallingRoger I Ceilley
Jan 27, 2010·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Abdel Kader El TalDavid A Mehregan
Jun 2, 2010·The American Journal of Dermatopathology·L Frank GlassBasil S Cherpelis
Jun 21, 2011·Journal of the American Academy of Dermatology·Ali HendiLawrence E Gibson
Jun 24, 2011·The American Journal of Dermatopathology·Will H BlackLewis Frank Glass
Jul 18, 2012·Dermatologic Clinics·Andrea M HuiDaniel M Siegel
Oct 30, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jessica NewmanPeter K Lee

❮ Previous
Next ❯

Citations

Apr 25, 2017·International Journal of Dermatology·Matthew F HelmCatherine G Chung
May 1, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·M LambertiniE Dika
Feb 9, 2018·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Euphemia W MuShane A Meehan
Sep 12, 2020·Journal of Cutaneous Medicine and Surgery·Annie LiuAn-Wen Chan
Apr 20, 2021·Journal of the American Academy of Dermatology·Jonas A AdalsteinssonDésirée Ratner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.