Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the d and m serotypes of plum pox potyvirus

Phytopathology
T CandresseJ Dunez

Abstract

ABSTRACT Plum pox potyvirus (PPV) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. Monoclonal antibodies specific for the two major groups of isolates, represented by the D and M serotypes of the virus, have been obtained. Polymerase chain reaction (PCR)-based assays allowing the direct detection and differentiation of PPV isolates have also been developed. We now report on a large-scale comparison of these two typing approaches. The results obtained show an overall excellent correlation between the results obtained in indirect double-antibody sandwich enzyme-linked immunosorbent assay using PPV-D- and PPV-M-specific monoclonal antibodies and those derived from either specific PCR assays or restriction fragment length polymorphism analysis of PCR fragments. Without exception, all isolates reacting positively with the PPV-M-specific monoclonal antibody were found to belong to the M serotype using the PCR-based assays, while 51 out of 53 isolates recognized by the D-specific monoclonal antibodies belonged to the D serotype according to the PCR typing results. However, failure to react with a specific monoclonal antibody did not prove as effective a predictor of the ser...Continue Reading

Citations

Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·K PrabhaR K Jain
Feb 3, 2000·Journal of Biotechnology·J J López-MoyaJ A García
May 6, 2006·Molecular Plant-microbe Interactions : MPMI·Véronique DecroocqF Revers
Dec 22, 2010·Phytopathology·T CandresseM Cambra
Nov 10, 2001·Annual Review of Phytopathology·Robert R MartinC André Lévesque
Oct 22, 2010·Plant Cell Reports·Elisa Di Nicola-NegriVincenza Ilardi
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·P SáenzJ A García
Nov 5, 2015·Archives of Virology·Sergei ChirkovIrina Mitrofanova
Apr 24, 2012·Virus Research·Lukáš PredajňaM Glasa
May 12, 2010·Research in Microbiology·Marina BarbaFen Beed
Oct 9, 2007·Journal of Virological Methods·Graziella PasquiniHenryk Czosnek
Oct 10, 2013·Molecular Plant Pathology·J A GarcíaT Candresse
Jul 6, 2004·Journal of Virological Methods·William L SchneiderReid D Frederick
Dec 17, 2014·Journal of Agricultural and Food Chemistry·Valentina UsenikMojca Virscek Marn
Sep 11, 2016·Journal of the Science of Food and Agriculture·Valentina Usenik, Mojca Virscek Marn
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·J FletcherS A Tolin

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