Comparison of monolithic silica and polymethacrylate capillary columns for LC

Journal of Separation Science
Dana MoravcováJosef Planeta

Abstract

Organic polymer monolithic capillary columns were prepared in fused-silica capillaries by radical co-polymerization of ethylene dimethacrylate and butyl methacrylate monomers with azobisisobutyronitrile as initiator of the polymerization reaction in the presence of various amounts of porogenic solvent mixtures and different concentration ratios of monomers and 1-propanol, 1,4-butanediol, and water. The chromatographic properties of the organic polymer monolithic columns were compared with those of commercial silica-based particulate and monolithic capillary and analytical HPLC columns. The tests included the determination of H-u curves, column permeabilities, pore distribution by inversed-SEC measurements, methylene and polar selectivities, and polar interactions with naphthalenesulphonic acid test samples. Organic polymer monolithic capillary columns show similar retention behaviour to chemically bonded alkyl silica columns for compounds with different polarities characterized by interaction indices, Ix, but have lower methylene selectivities and do not show polar interactions with sulphonic acids. The commercial capillary and analytical silica gel-based monolithic columns showed similar selectivities and provided symmetrical ...Continue Reading

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Citations

Sep 15, 2014·Journal of Chromatography. a·Michał SzumskiBogusław Buszewski
Dec 4, 2014·Journal of Chromatography. a·Michał SzumskiBogusław Buszewski
Jul 13, 2006·Journal of Separation Science·Jirí UrbanPavel Jandera
Oct 27, 2007·Journal of Separation Science·Zdenka KucerováPavel Jandera
Jul 16, 2008·Journal of Separation Science·Jirí Urban, Pavel Jandera
Dec 31, 2010·Analytical and Bioanalytical Chemistry·Ivo NischangOliver Brüggemann
Dec 1, 2020·Analytical Chemistry·Meng DingQiuquan Wang

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