PMID: 9535503Apr 16, 1998Paper

Comparison of motility and membrane integrity to assess rat sperm viability

Reproductive Toxicology
C M VetterT R Skopek

Abstract

Rat sperm motility and membrane integrity were compared as endpoints for viability. Sperm motility was measured by computer-assisted semen analysis (CASA), whereas membrane integrity was assessed by flow cytometric analysis of sperm stained with two nucleic acid stains, SYBR-14 and propidium iodide. The two techniques were compared in experiments that examined sperm viability over time and by analysis of known mixtures of control and freeze/thaw-killed sperm. Results from the two approaches were quantitatively very similar. Sperm from rats treated with dibromoacetic acid (600 or 1200 mg/kg) or alpha-chlorhyrin (100 mg/kg) were also analyzed. Neither technique detected a treatment-related effect with dibromoacetic acid. CASA identified a significant decrease in sperm motility in alpha-chlorhyrin-treated rats, whereas flow cytometric analysis did not find a measureable change in sperm membrane integrity. Because decreases in sperm motility would be expected to directly affect fertility, CASA may be a more robust endpoint for risk assessment in reproductive toxicology studies than flow cytometric analysis of membrane integrity.

References

Jan 1, 1992·Reproductive Toxicology·G P TothM K Smith
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Citations

Dec 15, 2010·Zygote : the Biology of Gametes and Early Embryos·P ChrenekM Simon
Jul 16, 2005·Journal of Toxicology and Environmental Health. Part a·D P ArfstenK R Still
Feb 10, 2009·Journal of Toxicology and Environmental Health. Part a·D P ArfstenM Y-V Bekkedal
Apr 8, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ellen PaynterBoris Baer
Feb 14, 2006·Reproductive Toxicology·Rosy El RamyBrigitte Le Magueresse-Battistoni
Aug 13, 2002·International Journal of Toxicology·M S ChristianD M Creasy
Apr 1, 2006·Archives of Andrology·J H WuL Cao
Jun 15, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·J-C LuN-Q Lu
May 20, 2004·Reproductive Medicine and Biology·Masakatsu FujinokiSadao Yamaoka

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