Comparison of mouse embryo development in open and microdrop co-culture systems

Human Reproduction
R SherbahnR G Rawlins

Abstract

Co-culture with numerous cell lines has been shown to improve in-vitro embryo development. It is usually performed in open culture without an oil overlay, or in relatively large volumes of medium (e.g. 0.5 ml) under oil. We compared the efficacy of open and microdrop co-culture systems using human endometrial and tubal cell lines and mouse zygotes. Although the mean pH values of the media from the tubal cell cultures (both open and oil-covered) decreased significantly over 5 days of culture, this did not appear to impair embryo development. Both co-culture and microdrop culture significantly improved blastocyst and hatching blastocyst formation rates. The combination of the two techniques (microdrop and co-culture) demonstrated the highest blastocyst formation and hatching blastocyst formation rates, as well as the highest mean cell numbers in hatching blastocysts. Co-culture in a microdrop is a superior system for mouse embryo culture.

Citations

Mar 13, 2010·In Vitro Cellular & Developmental Biology. Animal·Behrouz AflatoonianHarry Moore
Aug 5, 2009·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A V KarnaukhovL M Chailakhyan
Apr 21, 2005·Journal of Experimental & Clinical Assisted Reproduction·Mohamad Reza Baghaban Eslami NejadSaeed Kazemi Ashtiani
Apr 22, 2003·In Vitro Cellular & Developmental Biology. Animal·Hsin-Yang LiYen-Jen Sung
Nov 23, 2006·Theriogenology·Nicolas M Orsi, Judith B Reischl
Feb 14, 2014·Reproductive Medicine and Biology·Manami NishioEimei Sato
Dec 16, 2006·Reproductive Biomedicine Online·Poopak Eftekhari-YazdiLeila Karimian

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