Comparison of mouse mammary gland imaging techniques and applications: reflectance confocal microscopy, GFP imaging, and ultrasound.

BMC Cancer
Maddalena T TilliPriscilla A Furth

Abstract

Genetically engineered mouse models of mammary gland cancer enable the in vivo study of molecular mechanisms and signaling during development and cancer pathophysiology. However, traditional whole mount and histological imaging modalities are only applicable to non-viable tissue. We evaluated three techniques that can be quickly applied to living tissue for imaging normal and cancerous mammary gland: reflectance confocal microscopy, green fluorescent protein imaging, and ultrasound imaging. In the current study, reflectance confocal imaging offered the highest resolution and was used to optically section mammary ductal structures in the whole mammary gland. Glands remained viable in mammary gland whole organ culture when 1% acetic acid was used as a contrast agent. Our application of using green fluorescent protein expressing transgenic mice in our study allowed for whole mammary gland ductal structures imaging and enabled straightforward serial imaging of mammary gland ducts in whole organ culture to visualize the growth and differentiation process. Ultrasound imaging showed the lowest resolution. However, ultrasound was able to detect mammary preneoplastic lesions 0.2 mm in size and was used to follow cancer growth with seria...Continue Reading

References

Sep 1, 1981·The Histochemical Journal·A FraschiniM G Manfredi Romanini
Dec 29, 1998·Mechanisms of Development·A K HadjantonakisA Nagy
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M YangR M Hoffman
Oct 26, 2000·Academic Radiology·K W FerraraS A Wickline
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M YangR M Hoffman
Jan 10, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Alicia A LacyRebecca Richards-Kortum
Mar 21, 2002·Nature Reviews. Cancer·Ralph Weissleder
Jul 2, 2002·Clinical & Experimental Metastasis·Xiaoming LiRobert M Hoffman
Oct 31, 2002·Molecular Endocrinology·Valérie Gouon-Evans, Jeffrey W Pollard
Mar 12, 2003·Genes & Development·Tarik F Massoud, Sanjiv S Gambhir
Jul 4, 2003·The Journal of Nutrition·Claudine Kavanaugh, Jeffrey E Green
Nov 7, 2003·Cancer·Rebekah A DrezekMichele Follen
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meng YangRobert M Hoffman
Dec 18, 2003·Clinics in Dermatology·Salvador GonzálezAbel Torres
Jan 31, 2004·Technology in Cancer Research & Treatment·Konstantin SokolovAnn Gillenwater
Feb 20, 2004·Cancer Research·Pritha RaySanjiv S Gambhir
Mar 19, 2004·Nature·Hans-Guido WendelScott W Lowe
Oct 27, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vanessa Campo-RuizSalvador González
Nov 26, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Robert M Hoffman
Dec 8, 2004·Journal of the American Academy of Dermatology·Sarita NoriSalvador González
Jan 11, 2005·The Journal of Pathology·Scott K Lyons
Apr 28, 2005·Current Medicinal Chemistry·Byron BallouAlan S Waggoner
Oct 1, 2005·Nature Reviews. Cancer·Robert M Hoffman
Oct 21, 2006·Journal of Mammary Gland Biology and Neoplasia·John M BooneThomas R Nelson
Nov 4, 2006·Journal of Mammary Gland Biology and Neoplasia·Chandra M SehgalEmily F Conant
Nov 8, 2006·Journal of Mammary Gland Biology and Neoplasia·Lewis A Chodosh, Robert D Cardiff
Nov 9, 2006·Journal of Mammary Gland Biology and Neoplasia·Stavros G DemosAmir H Gandjbakhche
Nov 9, 2006·Journal of Mammary Gland Biology and Neoplasia·Craig K AbbeySimon R Cherry
Nov 16, 2006·Journal of Mammary Gland Biology and Neoplasia·Mazen SidaniJohn Condeelis
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Laura CiarloniCathrin Brisken

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Citations

Nov 26, 2009·Journal of Mammary Gland Biology and Neoplasia·John D LanduaMichael T Lewis
Aug 16, 2011·Cancer Research·Edgar S Díaz-CruzPriscilla A Furth
Aug 18, 2012·Stem Cells·Natesh ParashuramaSanjiv S Gambhir

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Methods Mentioned

BETA
confocal microscopy
biopsy
xenograft
imaging technique
transgenic
imaging techniques

Software Mentioned

Metamorph
Volume Viewer
VivaCell
VivaStacks
VS2000ui
ImageJ Analysis and Processing Java

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