Comparison of mRNA contents of interleukin-1 beta and nitric oxide synthase in pancreatic islets isolated from female and male nonobese diabetic mice

Diabetologia
M WelshStellan Sandler

Abstract

Interleukin-1 beta (IL-1 beta) has been suggested to mediate beta-cell destruction in insulin-dependent diabetes mellitus (IDDM) by inducing nitric oxide production. In this study, we assessed the levels of IL-1 beta and the inducible form of nitric oxide synthase (iNOS), using a semi-quantitative polymerase chain reaction assay, and performed determinations of nitrite accumulation and IL-1 beta bioactivity, on pancreatic islets isolated from 5- and 16-week-old female and male nonobese diabetic (NOD) mice and from nondiabetes prone NMRI mice. NOD mouse islets contained notable amounts of IL-1 beta mRNA. At 5 weeks of age, but not at 16 weeks, the values were higher in islets isolated from NOD females compared to males. The IL-1 beta bioactivity showed differences roughly reflecting the mRNA levels in the NOD mouse islets. In the NMRI mouse islets the IL-1 beta bioactivity was very low. The expression of iNOS mRNA increased in both male and female islets between 5 and 16 weeks of age. Immunocytochemistry of pancreatic sections indicated the presence of macrophages especially in the peri-insular area of the NOD mice which suggests that IL-1 beta was produced by macrophages. The levels of IL-1 beta activity and mRNA in freshly iso...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Nov 1, 1991·The Journal of Clinical Investigation·W P Arend
Sep 15, 1986·The American Journal of Cardiology·J AhlnerL Wallentin
Jan 1, 1971·Analytical Biochemistry·R T Hinegardner
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A F GazdarV Lauris
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 16, 1993·European Journal of Pharmacology·T P MiskoM G Currie
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A CorbettE R Unanue
May 1, 1993·Immunology Today·P PozzilliP E Beales

❮ Previous
Next ❯

Citations

Aug 10, 2000·Diabetes/metabolism Research and Reviews·H E Thomas, T W Kay
Jun 30, 2000·Biochemical and Biophysical Research Communications·T TabatabaieY Kotake
Sep 1, 1996·Diabetologia·T Mandrup-Poulsen
Aug 9, 2002·Upsala Journal of Medical Sciences·A King
Apr 11, 2008·Scandinavian Journal of Immunology·S HässlerO Winqvist
Dec 25, 2003·Diabetes·Helen E ThomasThomas W H Kay
Feb 9, 2005·Molecular & Cellular Proteomics : MCP·Thomas SparreFlemming Pociot
Oct 22, 2014·Cell Transplantation·Cheng-Yu LinHwai-Shi Wang
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Ragnar HenningssonIngmar Lundquist
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rima DarwicheThomas W H Kay
May 13, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vinicius N MottaJayne S Danska
Nov 30, 2000·Upsala Journal of Medical Sciences·S SandlerN Welsh
Nov 1, 1995·Clinical and Experimental Immunology·I BergerotC Thivolet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.