Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of carbapenemases producing Acinetobacter baumannii isolated from burn patients

Burns : Journal of the International Society for Burn Injuries
Leila AzimiAbdolaziz Rastegar Lari

Abstract

Acinetobacter baumannii is one of the important causes of nosocomial infection especially in burn patients. So, carbapenemase producing strains can make serious therapeutic problems. Molecular epidemiology studies play key role in decreasing the incidence of carbapenemase producing strains. In this study, 50 carbapenem resistant A. baumannii isolates from burn patients were collected. Antibiotic susceptibility testing and PCR assay was performed for detection of blaVIM, blaIMP, blaOXA-23,blaOXA-48,blaNDM-1, blaSPM-1, blaOxa-51 and blaKPC genes. Molecular typing for carbapenemase producing strains were performed by PFGE and MLVA. According to the results of antibiotic susceptibility tests all of strains were XDR and 46 (92%) harbored carbapenemase genes. PFGE and MLVA results showed the presence of 11 and 5 dominant clonal types respectively. The results of this study were showed the presence of certain clonal groups in two different wards of hospital indicating the spread of carbapenemase producing A. baumannii. On the other hand, the results showed the more discriminating power for PFGE.

References

Oct 12, 2000·Burns : Journal of the International Society for Burn Injuries·A R Lari, R Alaghehbandan
Dec 29, 2011·Dermatology Research and Practice·Leila AzimiAbdolaziz Rastegar Lari

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Citations

Nov 5, 2016·Diagnostic Microbiology and Infectious Disease·J Kristie JohnsonKerri A Thom
May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Apr 13, 2019·International Journal of Molecular Sciences·Li WangFaqin Dong
Oct 25, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L E PiriiE Bathoorn

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