Comparison of myelination between large and small pig fetuses during late gestation

Animal Reproduction Science
J L Vallet, J R Miles

Abstract

We compared myelination of the cerebellum, brain stem, and spinal cord in the largest and smallest pig fetuses within a litter during late gestation. Gilts were killed on Days 92, 100, and 110 of gestation and these neural tissues were obtained from the largest and smallest fetuses in each litter. Myelin basic protein (MBP) mRNA was quantified in each tissue using real time reverse transcriptase polymerase chain reaction (rtPCR). Myelin was recovered from each tissue and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and thin layer chromatography (TLC) was used to measure MBP and lipids, respectively. MBP mRNA increased with advancing gestation in all three tissues examined (P≤0.05) and was less in brain stem of small piglets compared to large piglets (P<0.01). Two coomassie stained protein bands (HMBP and LMBP) were observed by SDS-PAGE. Six prominent lipid bands were obtained by TLC (cholesterol, hydroxy(h)-cerebroside, nonhydroxy(nh)-cerebroside, phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin). Significant day by fetal size interactions for cerebellar MBP and lipids indicated that cerebellar myelination in the smallest fetuses was less compared to the largest fetuses on Days 100 and 11...Continue Reading

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Citations

Aug 8, 2015·Tropical Animal Health and Production·Morakot Nuntapaitoon, Padet Tummaruk
Jul 22, 2017·Asian-Australasian Journal of Animal Sciences·Morakot NuntapaitoonPadet Tummaruk
Feb 11, 2021·Animals : an Open Access Journal From MDPI·Kevin Van TichelenChris Van Ginneken

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