PMID: 8939400Dec 1, 1996Paper

Comparison of new solid-phase extraction methods for chromatographic identification of drugs in clinical toxicological analysis

Clinical Biochemistry
F Degel

Abstract

Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-phase column technology, their use in general screening and selective extraction methodology is discussed. Recovery studies were performed with urine-based samples including model substances, as well as drugs and metabolites from native urine samples. Chromatographic performance of the resulting extracts is presented in some examples. Liquid/liquid extraction, common mixed-phase column technique and the new SPEC-Plus, Multi-Modal disc extraction method gave the best recoveries with respect to broad-spectrum general screening. The purity of the concentrates was somewhat different, however. Solid-phase C18 and Solid-Phase Micro Extraction methodologies are better suited for selective drug extraction. In SPEC Disc or SPME extraction, the use of solvents is greatly reduced or omitted. Recovery of some volatile substances is enhanced. SPEC discs can be inserted directly into TLC chromatographic plates, SPME fibers into GC injection ...Continue Reading

References

Jan 1, 1991·Forensic Science International : Synergy·A Benkö, K Kimura
Dec 9, 1983·Journal of Chromatography·F T Delbeke, M Debackere
May 1, 1983·Journal of Analytical Toxicology·B FordT R Watson

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Citations

Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J P Franke, R A de Zeeuw
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A Polettini
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·O H Drummer
Dec 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·David Yong-Hoi YeungElizabeth Kwong
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Fritz PragstBjörn-Thoralf Erxleben
Dec 25, 2012·Bioprocess and Biosystems Engineering·Géraldine Le GoffJamal Ouazzani
Oct 19, 2006·Journal of Biochemical and Biophysical Methods·Elena E Stashenko, Jairo R Martínez
Dec 15, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Xiao-yan LiHai-feng Wang

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