Abstract
The EuropeanAspergillusPCR Initiative (EAPCRI) has provided recommendations for the PCR testing of whole blood (WB) and serum/plasma. It is important to test these recommended protocols on nonsimulated "in vivo" specimens before full clinical evaluation. The testing of an animal model of invasive aspergillosis (IA) overcomes the low incidence of disease and provides experimental design and control that is not possible in the clinical setting. Inadequate performance of the recommended protocols at this stage would require reassessment of methods before clinical trials are performed and utility assessed. The manuscript describes the performance of EAPCRI protocols in an animal model of invasive aspergillosis. Blood samples taken from a guinea pig model of IA were used for WB and serum PCR. Galactomannan and β-d-glucan detection were evaluated, with particular focus on the timing of positivity and on the interpretation of combination testing. The overall sensitivities for WB PCR, serum PCR, galactomannan, and β-d-glucan were 73%, 65%, 68%, and 46%, respectively. The corresponding specificities were 92%, 79%, 80%, and 100%, respectively. PCR provided the earliest indicator of IA, and increasing galactomannan and β-d-glucan values w...Continue Reading
References
Feb 28, 1996·JAMA : the Journal of the American Medical Association·P A NuttingS Holcomb
May 22, 1998·Statistics in Medicine·R G Newcombe
Oct 7, 2005·Journal of Clinical Microbiology·Laurence MillonStéphane Bretagne
Jan 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Lewis WhiteRosemary A Barnes
May 5, 2006·Journal of Clinical Microbiology·Monique A S H Mennink-KerstenPaul E Verweij
May 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ben De PauwUNKNOWN National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group
Oct 28, 2008·Archives of Toxicology·Akiko FuruyamaSeishiro Hirano
Dec 24, 2008·Journal of Clinical Pathology·R A BarnesJ Kell
Mar 14, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Catherine CordonnierMichaël Schwarzinger
Feb 12, 2010·Journal of Clinical Microbiology·P Lewis WhiteUNKNOWN European Aspergillus PCR Initiative
Sep 28, 2010·Bone Marrow Transplantation·Y MeijeM Cuenca-Estrella
Jan 14, 2011·Journal of Clinical Microbiology·L MillonE Deconinck
Sep 24, 2011·Journal of Clinical Microbiology·P Lewis WhiteUNKNOWN European Aspergillus PCR Initiative (EAPCRI)
Dec 23, 2011·Journal of Clinical Microbiology·Martina LengerovaThomas F Patterson
Jan 14, 2012·The Journal of Antimicrobial Chemotherapy·William R KirkpatrickThomas F Patterson
May 18, 2012·Journal of Clinical Microbiology·Jan SpringerJuergen Loeffler
Aug 23, 2012·Nature Immunology·Sarah E Hardison, Gordon D Brown
Feb 8, 2013·Clinical and Vaccine Immunology : CVI·P Lewis WhiteRosemary A Barnes
Feb 22, 2013·Journal of Clinical Microbiology·Jan SpringerP Lewis White
May 4, 2013·The Lancet Infectious Diseases·C Orla MorrisseyUNKNOWN Australasian Leukaemia Lymphoma Group and the Australia and New Zealand Mycology Interest Group
May 7, 2013·The Journal of Infection·Rosemary A BarnesP Lewis White
Oct 23, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·José María AguadoUNKNOWN Spanish Network for Research in Infectious Diseases
Apr 17, 2015·Journal of Clinical Microbiology·R L GortonC C Kibbler
Jun 19, 2015·Journal of Clinical Microbiology·Juergen LoefflerUNKNOWN European Aspergillus PCR Initiative
Jun 19, 2015·Journal of Clinical Microbiology·P Lewis WhiteUNKNOWN EAPCRI
Jun 27, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Lewis WhiteJ Peter Donnelly
Jul 15, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marios ArvanitisEleftherios Mylonakis
Citations
Sep 24, 2011·Journal of Perianesthesia Nursing : Official Journal of the American Society of PeriAnesthesia Nurses·Carol KahreRebecca P Winsett
Nov 24, 2016·The Journal of Antimicrobial Chemotherapy·Johan A MaertensPatricia Muñoz
Nov 1, 2017·Medical Mycology·Rosemary A BarnesJ Peter Donnelly
Nov 10, 2017·Journal of Clinical Microbiology·P Lewis WhiteRosemary A Barnes
Mar 27, 2018·Mycoses·Helena HammarströmVanda Friman
Feb 6, 2020·Journal of Fungi·Matthias EggerJuergen Prattes
Mar 30, 2017·The Journal of Antimicrobial Chemotherapy·Toine Mercier, Johan Maertens
Jul 12, 2019·Medical Mycology·P Lewis White
Jun 1, 2017·Frontiers in Microbiology·Guillaume Desoubeaux, Carolyn Cray
Feb 13, 2021·Journal of Fungi·Zoe Freeman WeissSophia Koo
Mar 13, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Lewis WhiteJohn R Wingard
Dec 23, 2021·Internal Medicine Journal·Abby P DouglasUNKNOWN Australasian Antifungal Guidelines Steering Committee