Comparison of Nonculture Blood-Based Tests for Diagnosing Invasive Aspergillosis in an Animal Model.

Journal of Clinical Microbiology
P Lewis WhiteThomas F Patterson

Abstract

The EuropeanAspergillusPCR Initiative (EAPCRI) has provided recommendations for the PCR testing of whole blood (WB) and serum/plasma. It is important to test these recommended protocols on nonsimulated "in vivo" specimens before full clinical evaluation. The testing of an animal model of invasive aspergillosis (IA) overcomes the low incidence of disease and provides experimental design and control that is not possible in the clinical setting. Inadequate performance of the recommended protocols at this stage would require reassessment of methods before clinical trials are performed and utility assessed. The manuscript describes the performance of EAPCRI protocols in an animal model of invasive aspergillosis. Blood samples taken from a guinea pig model of IA were used for WB and serum PCR. Galactomannan and β-d-glucan detection were evaluated, with particular focus on the timing of positivity and on the interpretation of combination testing. The overall sensitivities for WB PCR, serum PCR, galactomannan, and β-d-glucan were 73%, 65%, 68%, and 46%, respectively. The corresponding specificities were 92%, 79%, 80%, and 100%, respectively. PCR provided the earliest indicator of IA, and increasing galactomannan and β-d-glucan values w...Continue Reading

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Citations

Sep 24, 2011·Journal of Perianesthesia Nursing : Official Journal of the American Society of PeriAnesthesia Nurses·Carol KahreRebecca P Winsett
Nov 24, 2016·The Journal of Antimicrobial Chemotherapy·Johan A MaertensPatricia Muñoz
Nov 1, 2017·Medical Mycology·Rosemary A BarnesJ Peter Donnelly
Feb 6, 2020·Journal of Fungi·Matthias EggerJuergen Prattes
Mar 30, 2017·The Journal of Antimicrobial Chemotherapy·Toine Mercier, Johan Maertens
Jun 1, 2017·Frontiers in Microbiology·Guillaume Desoubeaux, Carolyn Cray
Dec 23, 2021·Internal Medicine Journal·Abby P DouglasUNKNOWN Australasian Antifungal Guidelines Steering Committee

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Methods Mentioned

BETA
PCR
PCRs
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism

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