Comparison of normal and abnormal fertilization of in vitro-matured human oocyte according to insemination method

The Journal of Obstetrics and Gynaecology Research
Ju Hee ParkSeok Hyun Kim

Abstract

Our purpose was to compare the normal fertilization rate, multi-pronuclei (PN) formation rate, and embryonic development of in vitro-matured oocytes between conventional insemination and intracytoplasmic sperm injection (ICSI). A total of 213 stimulated in vitro fertilization (IVF) cycles were selected, in which at least one immature oocyte was obtained (from 2010 to 2014). Immature oocytes were assigned to germinal vesicle (GV)-stage or metaphase I (MI)-stage oocyte groups. Cycles with obligatory ICSI due to male-factor infertility were excluded. Cycles were divided into two groups according to fertilization method: there were 97 cycles with conventional insemination and 116 cycles with ICSI. After in vitro maturation of 324 GV-stage oocytes and 341 MI-stage oocytes, the fertilization rate, multi-PN formation rate, and embryonic development were compared according to the fertilization method. The normal fertilization rate was similar in the conventional insemination and the ICSI both in GV-derived and MI-derived oocytes. Both fertilization methods resulted in a similar multi-PN formation rate in GV-derived oocytes; however, in MI-derived oocytes, the multi-PN formation rate was zero with ICSI and this was significantly lower t...Continue Reading

References

Mar 7, 2001·The Journal of Clinical Endocrinology and Metabolism·M FilicoriS Boschi
Feb 8, 2005·Human Reproduction·Viveca Söderström-AnttilaAnne-Maria Suikkari
May 8, 2007·Fertility and Sterility·Byung Chul JeeUNKNOWN Seoul National University College of Medicine Assisted Reproductive Technology (SMART) Study Group
Dec 27, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Pilar CoyManuel Aviles
Apr 3, 2009·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Raffaella DepaloLuigi Selvaggi
Apr 25, 2013·Clinical and Experimental Reproductive Medicine·Seung Bi ShinHyoung-Song Lee
May 6, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ju Yeong KimSeok Hyun Kim
Jun 12, 2014·Seminars in Reproductive Medicine·Samir N BabayevOrhan Bukulmez
Jan 21, 2015·JAMA : the Journal of the American Medical Association·Sheree L BouletDenise J Jamieson

❮ Previous
Next ❯

Citations

Jun 25, 2020·Journal of Assisted Reproduction and Genetics·E BoschUNKNOWN Spanish Infertility SWOT Group (SISG)
Nov 7, 2020·Journal of Assisted Reproduction and Genetics·Mounia HaddadGianpiero D Palermo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.