Comparison of novel and existing tools for studying drug sensitivity against the hookworm Ancylostoma ceylanicum in vitro

Parasitology
Lucienne TrittenJennifer Keiser

Abstract

The motility assay is the current gold standard for evaluating drug effects on hookworm larvae and adults, however, among other drawbacks the assay is time consuming, and prone to individual subjectivity. We evaluated six alternative in vitro assays, namely the feeding inhibition assay, the colourimetric AlamarBlue®, MTT formazan and acid phosphatase activity assays, as well as isothermal calorimetry and the xCELLigence System using Ancylostoma ceylanicum third-stage larvae, stimulated third-stage larvae and adults. The performances of the assays were compared to the motility assay using three standard drugs: albendazole, levamisole and ivermectin (100-1 μg/ml). None of the assays investigated offered an advantage over the motility assay, because they were all inapplicable to third-stage larvae, which were presumably metabolically and physically too inactive. Among all assays tested the xCELLigence System performed best on adult worms as the test was accurate, simple, required a minimal number of worms and offered the possibility for conducting a medium-throughput screening.

References

Aug 1, 1992·Experimental Parasitology·J M Hawdon, G A Schad
Nov 1, 1991·International Journal for Parasitology·J H GillE Lacey
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·P C Emson
Dec 1, 1981·Journal of Helminthology·A MisraJ C Katiyar
Oct 1, 1995·International Journal for Parasitology·J C RichardsI R Duce
Jul 1, 1997·The Veterinary Journal·R J Martin
Nov 13, 2001·Tropical Medicine & International Health : TM & IH·S Geerts, B Gryseels
Dec 26, 2001·Veterinary Parasitology·M A TaylorK L Goodyear
Sep 15, 2004·Trends in Parasitology·Adrian J WolstenholmeNicholas C Sangster
Sep 15, 2004·Trends in Parasitology·Ray M Kaplan
Dec 18, 2004·Advances in Parasitology·Simon BrookerPeter J Hotez
Feb 22, 2005·Chemical Research in Toxicology·James Zan XingXiao Xu
Jun 28, 2005·Molecular and Biochemical Parasitology·Jennifer M MoserGreg Gibson
Oct 14, 2005·Experimental Gerontology·Ann M BurnellJacques R Vanfleteren
Oct 24, 2007·Annals of the New York Academy of Sciences·Peter Hotez
Apr 3, 2008·The Journal of Clinical Investigation·Peter J HotezJulie Jacobson
Nov 10, 2009·FEMS Microbiology Letters·Olivier BraissantAlma U Daniels
Jan 30, 2010·Expert Review of Anti-infective Therapy·Michael O HarhayPiero L Olliaro
Jun 4, 2010·PLoS Neglected Tropical Diseases·Peter J Hotez, Bernard Pecoul
Jul 16, 2010·Advances in Parasitology·Jennifer Keiser, Jürg Utzinger
Jan 29, 2011·Journal of Clinical Microbiology·Theresia ManneckJennifer Keiser
Feb 15, 2011·Acta Tropica·Carla KirchhoferJennifer Keiser
Sep 6, 2011·Journal of Microbiological Methods·Angelika SilbereisenJennifer Keiser

❮ Previous
Next ❯

Citations

Feb 26, 2013·Parasites & Vectors·David WimmersbergerJennifer Keiser
Nov 1, 2015·Applied Microbiology and Biotechnology·Dayana FloresJennifer Keiser
Aug 20, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Gabriel RinaldiMichael J Smout
Apr 16, 2013·Parasite Immunology·R A Paveley, Q D Bickle
Apr 14, 2015·BioMed Research International·Sebastião Rodrigo FerreiraRicardo Toshio Fujiwara
Apr 29, 2014·International Journal for Parasitology·Ana E ZeraikPaul J Brindley
Oct 19, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Janis C WeeksJohn M Hawdon
Jun 20, 2018·Antimicrobial Agents and Chemotherapy·Noemi HiroshigeJennifer Keiser
Sep 9, 2021·Clinical Microbiology Reviews·Oyelola AdegboyeClaire Loiseau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.