PMID: 3754396Feb 15, 1986Paper

Comparison of nucleic acid-protein interactions in solution and in polyacrylamide gels

Analytical Biochemistry
A RevzinM M Garner

Abstract

The properties of nucleic acid-protein complexes in polyacrylamide gels and in solution have been compared. Transcription by RNA polymerase from a lac DNA template within a gel yields products which are the same as those obtained in solution. Likewise the dissociation rates of DNA-protein complexes in a gel are found to be identical to the solution values. Thus complexes existing in bands during gel electrophoresis behave quite normally. There seems to be no "caging" effect, at least not in a typical 5% polyacrylamide gel (30:1, acrylamide: bisacrylamide); the gel matrix does not hinder separation of DNA and protein molecules which have dissociated.

References

Dec 28, 1966·Journal of Molecular Biology·O W Jones, P Berg
Jan 25, 1984·Journal of Molecular Biology·M G Fried, D M Crothers

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Citations

Oct 1, 1996·Electrophoresis·B P Belotserkovskii, B H Johnston
Jul 31, 2001·International Journal of Cancer. Journal International Du Cancer·J P SteinbachA Aguzzi
Dec 28, 1994·Journal of Immunological Methods·S Y StevensG D Glick
Jul 19, 2000·Trends in Biotechnology·K A XavierT Giordano
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R W KriwackiJ P Caradonna
Jun 25, 1995·Nucleic Acids Research·K M Vossen, M G Fried
Jan 3, 2012·Nucleic Acids Research·Gregory P DonaldsonVincent T Lee
Jun 15, 1987·European Journal of Biochemistry·R ImberU Heinemann
Dec 26, 1986·Cell·H N Liu-JohnsonD M Crothers
May 1, 1989·Electrophoresis·J A Ceglarek, A Revzin
Dec 1, 1992·Microbiological Reviews·D LaneM Chandler

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