Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells.

The Journal of Biological Chemistry
K O LloydJ Taylor-Papadimitriou

Abstract

MUC-1 mucin is considered to be aberrantly glycosylated in breast, ovary, and other carcinomas in comparison with mucin from corresponding normal tissues. In order to clarify these differences in glycosylation, we have compared the O-linked carbohydrate chains from MUC-1 immunoprecipitated from [3H]GlcN-labeled breast epithelial cell lines (MMSV1-1, MTSV1-7, and HB-2) derived from cells cultured from human milk, with three breast cancer cell lines (MCF-7, BT-20, and T47D). Analysis by high pH anion chromatography showed that the normal cell lines had a higher ratio of GlcN/GalN and more complex oligosaccharide profiles than the cancer cell lines. Structural analyses were carried out on the oligosaccharides from MTSV1-7 and T47D MUC-1, and the following structures were proposed. MUC-1 from T47D had rather a simple glycosylation pattern, with NeuAcalpha2-3Galbeta1-3GalNAc-ol, Galbeta1-3GalNAc-ol, and GalNAc-ol predominating; in contrast, MUC-1 from MTSV1-7 had more complex structures, including a number of disialo, core 2 species, i.e. NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-6[NeuAcalpha2 -3Galbeta1-3]GalNAc- ol and NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-6[NeuAcalpha2 -3Galbeta1-4GlcNAcbet a1-3Galbeta1-3]GalNAc-ol. Double-labeling experim...Continue Reading

References

May 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I KeydarH J Brenner
May 29, 1992·Biochemical and Biophysical Research Communications·L PembertonS J Gendler
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J BartekJ Taylor-Papadimitriou
Aug 19, 1991·International Journal of Cancer. Journal International Du Cancer·J Taylor-Papadimitriou
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·G J Strous, J Dekker
Sep 1, 1991·The American Review of Respiratory Disease·S J GendlerJ Taylor-Papadimitriou
Jan 1, 1990·Glycoconjugate Journal·F G HanischG Uhlenbruck
Dec 31, 1990·Biochemical and Biophysical Research Communications·C A LancasterS J Gendler
Jun 15, 1989·International Journal of Cancer. Journal International Du Cancer·A GirlingJ Taylor-Papadimitriou
Nov 1, 1973·Journal of the National Cancer Institute·H D SouleM Brennan
Jan 1, 1971·Archives of Biochemistry and Biophysics·R N Iyer, D M Carlson
Jan 1, 1982·Methods in Enzymology·K YamashitaA Kobata
Jul 15, 1981·International Journal of Cancer. Journal International Du Cancer·J Taylor-PapadimitriouW F Bodmer
Jan 1, 1994·Advances in Experimental Medicine and Biology·P X XingI F McKenzie
Oct 15, 1995·European Journal of Biochemistry·I BrockhausenJ Taylor-Papadimitriou
Aug 12, 1993·Annals of the New York Academy of Sciences·J Taylor-PapadimitriouP Beverley
Jan 1, 1995·Annual Review of Physiology·S J Gendler, A P Spicer
Nov 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C PetrarcaM Nuti

❮ Previous
Next ❯

Citations

May 16, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·E DrakopoulouF Hudecz
Jul 6, 2001·The Journal of Pathology·M J HudsonE N Lalani
Jul 12, 2002·Rapid Communications in Mass Spectrometry : RCM·Yunping HuangMilos V Novotny
Jul 7, 2005·Pathology Oncology Research : POR·María V CroceAmada Segal-Eiras
Jul 4, 2007·Pathology Oncology Research : POR·María V CroceAmada Segal-Eiras
Aug 7, 2012·Cellular and Molecular Life Sciences : CMLS·Milada Stuchlová HorynováJan Novak
Feb 18, 2006·Cancer Immunology, Immunotherapy : CII·Antje DanielczykSteffen Goletz
Feb 21, 2007·Cancer Immunology, Immunotherapy : CII·Peter RavnSteffen Goletz
Dec 4, 2009·Cancer Immunology, Immunotherapy : CII·Christoph DomschkePhilipp Beckhove
Jun 21, 2005·Springer Seminars in Immunopathology·Mara Gerloni, Maurizio Zanetti
Mar 22, 2012·Seminars in Immunopathology·Jan NovakMatthew B Renfrow
Nov 22, 2011·Annals of Biomedical Engineering·Yue GengMichael R King
Jan 10, 2012·Clinical & Experimental Metastasis·Hidenori OzakiHisashi Narimatsu
Oct 11, 2005·Autoimmunity Reviews·Maurizio Zanetti
May 29, 2003·Biochimie·Ricardo Gutiérrez GallegoJohannes F G Vliegenthart
Sep 21, 2002·Molecular and Cellular Endocrinology·Vicenta Garcia-CampayoIrving Boime
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Nov 26, 1999·Biochimica Et Biophysica Acta·J Taylor-PapadimitriouM Dalziel
Aug 2, 2002·Bioorganic & Medicinal Chemistry·Constanze Brocke, Horst Kunz
Jul 18, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Andrew W HorneEl-Nasir Lalani
Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
Nov 11, 2009·Journal of the American Chemical Society·Naoki OhyabuShin-ichiro Nishimura
Jun 3, 2005·Nature Reviews. Drug Discovery·Danielle H Dube, Carolyn R Bertozzi
Feb 24, 2001·Journal of Gastroenterology and Hepatology·M NakagawaT Tsuji
Aug 9, 2012·The Journal of Biological Chemistry·Helene BayerCristina Voss
Mar 11, 2000·Molecular Biology of the Cell·Y AltschulerR P Hughey
Nov 24, 1999·Glycobiology·J BurchellJ Taylor-Papadimitriou
Apr 5, 2001·Journal of the National Cancer Institute·S GuruswamyD M Benbrook
Sep 22, 2001·Journal of Immunotherapy·K MaruyamaK Yamaguchi
Mar 1, 2003·Applied Immunohistochemistry & Molecular Morphology : AIMM·Julie Teruya-FeldsteinR S K Chaganti

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.