Comparison of ocular pathologies in vitamin A-deficient mice and RPE65 gene knockout mice.

Investigative Ophthalmology & Visual Science
Yang HuJian-Xing Ma

Abstract

RPE65 gene knockout (Rpe65⁻/⁻) mice showed abolished isomerohydrolase activity in the visual cycle and were considered a model for vitamin A deficiency in the retina. The purpose of this study was to compare the retinal phenotypes between vitamin A-deficient (VAD) mice and Rpe65⁻/⁻ mice under normal diet. The VAD mice were fed with a vitamin A-deprived diet after birth. The age-matched control mice and Rpe65⁻/⁻ mice were maintained under normal diet. The structure of photoreceptor outer segment was compared using electron microscopy. Photoreceptor-specific gene expression was determined using real-time RT-PCR. The isomerohydrolase and lecithin-retinol acyltransferase (LRAT) activities were measured using an in vitro enzymatic activity assay. Endogenous retinoid profiles were analyzed by HPLC in mouse eyecup homogenates. Compared to wild-type mice under normal diet, scanning and transmission electron microscopy showed that the outer segments of photoreceptors were disorganized in VAD mice and were not disorganized in Rpe65⁻/⁻ mice, although they were shortened in the latter. VAD mice showed more prominent downregulation of middle wavelength cone opsin, whereas Rpe65⁻/⁻ mice displayed more suppressed expression of short wavelengt...Continue Reading

Citations

Aug 1, 2013·Documenta Ophthalmologica. Advances in Ophthalmology·Tomas S AlemanAlexander J Brucker
Apr 28, 2020·Progress in Retinal and Eye Research·Johannes von LintigDarwin Babino
Jul 25, 2017·The American Journal of Pathology·Volha V MalechkaJian-Xing Ma
May 3, 2021·Journal of Cellular and Molecular Medicine·Joe M ButlerLuminita Paraoan

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