Comparison of oestrogen receptor assessment in frozen and paraffin sections

Pathology, Research and Practice
M FeeP Dervan

Abstract

Estimation of oestrogen receptors (ER) in breast carcinoma has important therapeutic and prognostic implications. The dextran-coated charcoal (DCC) biochemical technique is being replaced in many institutions by immunohistochemical techniques. The aim of this study was to assess the level of agreement between ER counts in frozen and paraffin sections. Ninety-seven infiltrating breast carcinomas were studied immunohistochemically, for ER in frozen and paraffin sections. Cases were classified as ER-positive if > or = 10% of the tumor cells were positive. Both techniques gave a high level of correlation and agreement. There was complete agreement in classifying cases as ER-positive or ER-negative. ER estimation on only one case was outside 2SD of complete agreement. Oestrogen-receptor content can be assayed satisfactorily in paraffin sections and offers many advantages over the frozen section technique.

References

Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Oct 1, 1989·European Journal of Cancer & Clinical Oncology·G SøndergaardS M Paulsen

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Citations

Jul 1, 1996·Irish Journal of Medical Science·T F Gorey
Apr 17, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Sharon Nofech-MozesWedad M Hanna
May 23, 2012·Clinical Oncology : a Journal of the Royal College of Radiologists·S Nofech-MozesW M Hanna
Jun 9, 2006·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D J HicksA Wangoo

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