Comparison of oil-in-water and water-in-oil microemulsion electrokinetic chromatography as methods for the analysis of eight phenolic acids and five diterpenoids

Electrophoresis
Jun CaoLian-Wen Qi

Abstract

Oil-in-water (O/W) and water-in-oil (W/O) MEEKC were compared for their abilities to separate and detect eight phenolic acids and five diterpenoids in Radix et Rhizoma Salviae Miltiorrhizae (RRSM). The effects of oil type and concentration, organic modifier, SDS, and buffer concentration on separation were examined in order to optimize the two methods. Oil contents and organic modifier were found to markedly influence the separation selectivity for both O/W and W/O systems. SDS concentration rarely affected separation resolution for O/W MEEKC, and separation of eight phenolic acids and five diterpenoids could be improved by changing the buffer concentration for W/O MEEKC. A highly efficient O/W MEEKC separation method, where the 13 compounds were separated with baseline resolution, was achieved by using a microemulsion solution of pH 8.0 containing 0.6% cyclohexane, 3.0% SDS, 6.0% 1-butanol, and 3.0% ACN. The W/O MEEKC was unable to resolve all the components. In addition, the analytic time in O/W MEEKC was shorter than that in W/O MEEKC. Finally, the developed O/W MEEKC method was successfully applied to determine analytic compounds in RRSM samples.

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Citations

Dec 21, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Heli SirénJohanna Suomi
Mar 29, 2011·Journal of Chromatography. a·Yuhua CaoJianwei Sheng
Dec 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Roberto Gotti
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Dec 21, 2010·Electrophoresis·Richie RyanKevin Altria
Mar 2, 2010·Electrophoresis·Richie RyanKevin Altria
May 3, 2016·Journal of Pharmaceutical and Biomedical Analysis·Lei LiuHua Yang
Jul 16, 2009·Journal of Agricultural and Food Chemistry·Guo-Xin ZhongShao-Ping Li

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