Comparison of oligonucleotide-labeled antibody probe assays for prostate-specific antigen detection

Analytical Biochemistry
Xuecheng JiangChenggang Zhu

Abstract

As a specific tumor marker, prostate-specific antigen (PSA) is widely used for the early diagnosis of prostate cancer. Sensitive and specific methods are required to improve the diagnostic accuracy of PSA detection. In the current study, we compared the immuno-polymerase chain reaction (immuno-PCR) method with the solid-phase proximity ligation assay (SP-PLA) with respect to the detection of PSA. Using oligonucleotide-labeled antibody probes, we used both immuno-PCR and SP-PLA to detect trace levels of PSA. The nucleic acid sequences can be monitored using real-time PCR. SP-PLA, however, was found to be superior in terms of both the detection limit and the dynamic range. To detect even lower levels of PSA, we used the loop-mediated isothermal amplification (LAMP) method to measure the levels of reporter DNA molecules in SP-PLA. The sensitivity of the LAMP method is 0.001 pM, which is approximately 100-fold higher than the sensitivities of the other assays. The results suggest that an SP-PLA- and LAMP-based protocol with oligonucleotide-labeled antibody probes may have great application in detecting PSA or other proteins present at trace levels.

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
May 1, 2002·Nature Biotechnology·Simon FredrikssonUlf Landegren
May 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mats GullbergSimon Fredriksson
Mar 23, 2005·Trends in Biotechnology·Christof M NiemeyerRon Wacker
Aug 23, 2005·Journal of Immunological Methods·Kristina Lind, Mikael Kubista
May 26, 2006·Clinical Chemistry·Sigrun M GustafsdottirUlf Landegren
Aug 4, 2007·Biotechnology Letters·Yan-Mei QiaoDian-Bing Wang
May 22, 2008·The Analyst·Michael AdlerChristof M Niemeyer
Jan 14, 2009·Journal of Virological Methods·Janet BarlettaNiel T Constantine
Dec 4, 2009·Molecular & Cellular Proteomics : MCP·Spyros DarmanisMasood Kamali-Moghaddam

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Citations

Dec 4, 2012·Biosensors & Bioelectronics·Honghong YinChuanlai Xu
Feb 14, 2016·Analytica Chimica Acta·Le ChangLunan Wang
Dec 1, 2012·Journal of Healthcare Engineering·Alicia D Powers, Sean P Palecek
Apr 15, 2016·Biomolecular Detection and Quantification·Christina GreenwoodStephen A Bustin
Oct 11, 2016·Molecular and Cellular Probes·Samine RahmatpourEsmaeil Sadroddiny
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·D Y RyazantsevS K Zavriev
Nov 8, 2017·Mikrochimica Acta·Dominika DamborskaJan Tkac
Nov 5, 2019·New Biotechnology·Giulio BernardinelliArio de Marco

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