Comparison of Oral Microbe Quantities from Tongue Samples and Subgingival Pockets

International Journal of Dentistry
André GöhlerBirte Holtfreter

Abstract

To improve understanding of periodontitis pathology, we need more profound knowledge of relative abundances of single prokaryotic species and colonization dynamics between habitats. Thus, we quantified oral microbes from two oral habitats to gain insights into colonization variability and correlation to the clinical periodontal status. We analyzed tongue scrapings and subgingival pocket samples from 237 subjects (35-54 years) with at least 10 teeth and no recent periodontal treatment from the 11-year follow-up of the Study of Health in Pomerania. Relative abundances of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Streptococcus sanguinis, total bacteria, and Archaea were correlated to clinically assessed pocket depths (PD) and clinical attachment levels (CAL). Increased relative abundances of P. gingivalis, A. actinomycetemcomitans, and F. nucleatum were linked to increased levels of PD and CAL (i) on the subject level (mean PD, mean CAL) and (ii) in subgingival pockets. Relative abundances of Archaea from tongue samples correlated negatively with mean PD or mean CAL. Detection and quantity of bacterial species correlated weakly to moderately between the tongue and subgingival pocket,...Continue Reading

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Citations

Mar 28, 2019·Frontiers in Cellular and Infection Microbiology·Yiping WeiXiaolei Wu
Nov 7, 2019·International Journal of Environmental Research and Public Health·Chie OmoriManabu Morita
Aug 21, 2018·International Journal of Dentistry·André GöhlerBirte Holtfreter
Aug 17, 2021·Oral Diseases·Ayat Gamal-AbdelNaserFat'heya M Zahran

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Methods Mentioned

BETA
scrapings
scraping

Software Mentioned

LightCycler 480

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