Comparison of osteogenic differentiation capacity in mesenchymal stem cells derived from human amniotic membrane (AM), umbilical cord (UC), chorionic membrane (CM), and decidua (DC)

Cell & Bioscience
Chongyang ShenHai Zhao

Abstract

Mesenchymal stem cells (MSCs) have been extensively explored as a promising therapeutic agent in the field of bone tissue engineering due to their osteogenic differentiation ability. In this study, the osteogenic differential ability and the effect of fibronectin and laminin on the osteogenic differentiation in four types of MSCs derived from placental tissue are compared to determine the ideal source for bone reconstruction tissue engineering. The present study examines the osteogenic differentiation levels of four types of MSCs using alizarin red staining and quantifies the calcium levels and alkaline phosphatase (ALP) activity. In addition, this study examines the osteoblast differentiation protein markers osterix, collagen I, osteopontin, and osteocalcin using a Western blot assay. qPCR and EdU labeling assays were employed to identify the kinetics of osteogenic differentiation. Calcium deposit levels, ALP activity, and osteopontin and osteocalcin concentrations were determined to confirm the role of Extracellular matrix (ECM) components role on the osteogenic differentiation of MSCs. The data demonstrated that MSCs isolated from different layers of placenta had different potentials to differentiate into osteogenic cells. I...Continue Reading

References

Oct 16, 2004·Journal of Cellular Biochemistry·Genevieve M BolandRocky S Tuan
Jul 16, 2008·Journal of Materials Science. Materials in Medicine·Shikhar VohraSusan L Bellis
Jun 20, 2015·Stem Cells International·Carla A PiccinatoEliane Antonioli
Mar 2, 2017·Tissue Engineering and Regenerative Medicine·Zongning MiaoYifeng Xue

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Citations

Nov 12, 2019·Journal of Biomedical Materials Research. Part a·Sevda P SevariAlireza Moshaverinia
Jan 23, 2021·Journal of Cellular Biochemistry·Angelica Mastandrea AmansoS L Goudy
Nov 3, 2020·Frontiers in Endocrinology·Jin LiYang Xia
Mar 20, 2021·Stem Cell Research & Therapy·Alexander MarkovAli Hassanzadeh
May 1, 2021·Reproductive Biology·Prasad Pethe, Vaijayanti Kale
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Vignesh K Manivasagam, Ketul C Popat

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Methods Mentioned

BETA
ELISA
fluorescence microscopy
protein assay

Software Mentioned

GraphPad Prism
GraphPad

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