Comparison of p16INK4a and ProEx C immunostaining on cervical ThinPrep cytology and biopsy specimens

Diagnostic Cytopathology
Trynda N ObergAmy C Clayton

Abstract

ProEx C and p16(INK4a) staining of cytology/histology specimens have recently been explored to help distinguish high-grade squamous intraepithelial lesions (HSIL) from benign mimics. The goal of this study was to evaluate the performance characteristics of p16 and ProEx C in tissue and patient matched ThinPrep liquid-based cytology specimens. Residual cervical ThinPrep cytology specimens and tissue blocks (N = 64) from 63 patients were stained with p16 and ProEx C. Review of immunostained material, Papanicolaou and H&E stained slides was performed by two cytopathologists. The cytology slides were evaluated for the presence or absence of squamous atypia as well as immunoreactivity. Histologic specimens were interpreted as negative, indeterminate, or positive for each immunostain. There was 86% agreement (55/64) between the p16 and ProEx C stains on tissue specimens. Eleven specimens were interpreted as positive for both stains. All had a low- or high-grade squamous lesion on the corresponding H&E section. ProEx C was able to identify four low-grade squamous intraepithelial lesion specimens that were interpreted as negative by p16. All four HSIL specimens demonstrated p16 and ProEx C staining. However, 84% of cytology negative sp...Continue Reading

References

Dec 8, 2004·Acta Cytologica·Marcus J TrunkMagnus von Knebel Doeberitz
Aug 4, 2005·Diagnostic Cytopathology·Liron PantanowitzChristopher N Otis
Mar 18, 2008·American Journal of Clinical Pathology·Maria BenevoloRaffaele Perrone Donnorso
Apr 22, 2008·The American Journal of Surgical Pathology·Riem E BadrShikha Bose
Jun 17, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Alvaro P PintoEdmund S Cibas
Oct 16, 2008·American Journal of Clinical Pathology·Momin T SiddiquiAziza Nassar

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Citations

Dec 2, 2011·Journal of Oncology·Sonya J Hwang, Kenneth R Shroyer
Dec 1, 2018·Archives of Gynecology and Obstetrics·Ferenc Zoltan TakacsMaximilian Linxweiler

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