PMID: 9542924May 23, 1998Paper

Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial

Journal of Clinical Microbiology
U M MorganR C Thompson

Abstract

PCR technology offers alternatives to conventional diagnosis of Cryptosporidium for both clinical and environmental samples. We compared microscopic examination by a conventional acid-fast staining procedure with a recently developed PCR test that can not only detect Cryptosporidium but is also able to differentiate between what appear to be host-adapted genotypes of the parasite. Examinations were performed on 511 stool specimens referred for screening on the basis of diarrhea. PCR detected a total of 36 positives out of the 511 samples, while routine microscopy detected 29 positives. Additional positives detected by PCR were eventually confirmed to be positive by microscopy. A total of five samples that were positive by routine microscopy at Western Diagnostic Pathology but negative by PCR and by microscopy in our laboratory were treated as false positives. Microscopy therefore exhibited 83.7% sensitivity and 98.9% specificity compared to PCR. PCR was more sensitive and easier to interpret but required more hands-on time to perform and was more expensive than microscopy. PCR, however, was very adaptable to batch analysis, reducing the costs considerably. Bulk buying of reagents and modifications to the procedure would decreas...Continue Reading

References

Dec 1, 1992·Journal of Clinical Microbiology·M N WidjojoatmodjoJ Verhoef
Sep 1, 1991·Applied and Environmental Microbiology·M W LeChevallierR G Lee
Feb 1, 1995·Journal of Clinical Microbiology·K S KehlP L Havens
Feb 1, 1995·International Journal for Parasitology·P J O'Donoghue
Apr 1, 1994·European Journal of Epidemiology·J Rodríguez-HernandezA M Martín-Sánchez
Jul 21, 1994·The New England Journal of Medicine·W R Mac KenzieJ B Rose
Jan 1, 1995·Trends in Genetics : TIG·J S Boyle, A M Lew
Nov 1, 1995·Applied and Environmental Microbiology·D W JohnsonJ B Rose
Apr 1, 1997·Journal of Clinical Microbiology·L MonteiroF Mégraud

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Citations

Feb 24, 2001·The Journal of Parasitology·U M MorganR C Thompson
Mar 21, 2001·Australian Veterinary Journal·K M DenholmP J Irwin
Sep 23, 2003·Infection and Immunity·Donna E AkiyoshiSaul Tzipori
Dec 6, 2005·Parasitology Research·Barbara MagiAngela Barberi
Aug 25, 2007·Annals of Tropical Medicine and Parasitology·N Abreu-AcostaB Valladares
Oct 14, 2009·Clinical Microbiology Reviews·D StarkJ T Ellis
Nov 28, 2012·The American Journal of Tropical Medicine and Hygiene·Julia den HartogWilliam A Petri
Feb 8, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·S A BhatN K Singh
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Marwa M I GhallabAyman A El-Badry
Feb 24, 1999·Australian Veterinary Journal·U M MorganR C Thompson
Aug 18, 2006·Journal of Medical Microbiology·Suzanne E StroupEric R Houpt
Jun 17, 2008·The Korean Journal of Parasitology·Sang-Mee GukMin Seo
Feb 26, 2011·The Journal of Parasitology·V R S FiuzaF C R Oliveira
Mar 30, 2013·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Flavio Medeiros Paz E SilvaJoão Pessoa Araújo-Junior
Feb 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beauty E OmoruyiFrancis O Okonkwo
Nov 6, 2014·Jundishapur Journal of Microbiology·Mehdi VejdaniAli Michaeli
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·S AghamolaieN Salehi
Feb 4, 2016·Journal of Water and Health·Abidelfatah M Nasser
Sep 1, 2016·Journal of Clinical Microbiology·Alessandro Rossi, Marc Roger Couturier
Aug 21, 2003·American Journal of Veterinary Research·Carey L KeithMichael R Lappin
Oct 7, 2006·Current Opinion in Gastroenterology·A Das, E P Variyam
Jun 6, 2006·Applied and Environmental Microbiology·Nihal T GodiwalaBrett A Leav
Dec 17, 2010·AIDS·Roberta M O'connorHonorine D Ward
Aug 22, 2012·Current Opinion in Infectious Diseases·Debbie-Ann T ShirleyKaren L Kotloff
Feb 9, 2012·Journal of Feline Medicine and Surgery·Francesca de Oliveira LemosNayro Xavier Alencar
Apr 4, 2014·Clinical Microbiology Reviews·Jaco J Verweij, C Rune Stensvold
Sep 25, 2010·Annals of Tropical Medicine and Parasitology·J YakoobW Jafri
Jul 1, 2019·Diagnosis·Albert Bernet SánchezMercè García González

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