Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever

Frontiers in Microbiology
M Gill HartleyT P Atkins

Abstract

Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viable bacterial load within tissues. Following recent advances in the culture methods, it has become possible to do the same with C. burnetii. Here we compare and contrast the different information gained by using PCR or viable counts to study this disease. Viable bacteria were cleared from organs much faster than previously reported when assessed by bacterial DNA, but weight loss and clinical signs improved while animals were still heavily infected.

References

Mar 1, 1987·Journal of General Microbiology·G H ScottE H Stephenson
Sep 12, 2000·Epidemiology and Infection·R J HarrisB P Marmion
Nov 23, 2006·Annals of the New York Academy of Sciences·M TurraM Qiao
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anders OmslandRobert A Heinzen
Nov 30, 2013·Journal of Microbiological Methods·Kelsi M SandozRobert A Heinzen
Jul 9, 2014·Antimicrobial Agents and Chemotherapy·I H NorvilleT P Atkins
Apr 22, 2015·Vector Borne and Zoonotic Diseases·Monika Szymańska-CzerwińskaJózef Piotr Knap
May 11, 2016·Infection and Immunity·Cléa MelenotteFabienne Brégeon
Feb 22, 2017·Frontiers in Cellular and Infection Microbiology·Erin J van SchaikJames E Samuel
Dec 7, 2017·Journal of Clinical and Diagnostic Research : JCDR·Jothimani PradeepDhandapany Gunasekaran
Jun 22, 2018·Current Protocols in Microbiology·Savannah E SanchezAnders Omsland

❮ Previous
Next ❯

Citations

Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Michelle NelsonTimothy P Atkins

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
RSA

Software Mentioned

AeroMP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon saikingaku zasshi. Japanese journal of bacteriology
H Oda, K Yoshiie
Journal of the American Veterinary Medical Association
D E Behymer, H P Riemann
DTW. Deutsche tierärztliche Wochenschrift
E H Schaal
© 2021 Meta ULC. All rights reserved