Comparison of pectic enzymes produced by Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, and Erwinia carotovora subsp. atroseptica.

Applied and Environmental Microbiology
J L Ried, A Collmer

Abstract

Erwinia spp. that cause soft-rot diseases in plants produce a variety of extracellular pectic enzymes. To assess the correlation between patterns of pectic enzyme production and taxonomic classification, we compared the enzymes from representative strains. Supernatants obtained from polygalacturonate-grown cultures of nine strains of Erwinia chrysanthemi, three strains of E. carotovora subsp. carotovora, and three strains of E. carotovora subsp. atroseptica were concentrated and subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. Pectate lyase, polygalacturonase, and exo-poly-alpha-D-galacturonosidase activities were visualized by staining diagnostically buffered pectate-agarose overlays with ruthenium red after incubation of the overlays with the isoelectric focusing gels. The isoelectric focusing profiles of pectate lyase and polygalacturonase were nearly identical for strains of E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, showing three pectate lyase isozymes with isoelectric points higher than 8.7 and a polygalacturonase with pI of ca. 10.2. Isoelectric focusing profiles of the E. chrysanthemi pectic enzymes were substantially different. Although there was considerable intraspecifi...Continue Reading

References

Oct 1, 1985·Journal of Bacteriology·D L Roeder, A Collmer
Mar 1, 1985·Applied and Environmental Microbiology·R T Zink, A K Chatterjee
Jan 1, 1984·Annual Review of Biochemistry·M McNeilP Albersheim

❮ Previous
Next ❯

Citations

Dec 1, 1990·Applied Biochemistry and Biotechnology·S FukuokaI Karube
Aug 31, 2011·PloS One·Maria del Pilar Marquez-VillavicencioAmy O Charkowski
Feb 3, 2016·Molecular Plant Pathology·Jarred Yasuhara-BellAnne M Alvarez
Jan 1, 1996·Annual Review of Microbiology·N Hugouvieux-Cotte-PattatS Reverchon
Nov 1, 1989·Molecular Microbiology·N Hugouvieux-Cotte-Pattat, J Robert-Baudouy
Feb 27, 1999·Journal of Bacteriology·V E ShevchikN Hugouvieux-Cotte-Pattat
Nov 25, 1988·Journal of Chromatography·T A McKeon
Dec 1, 1996·Journal of Bacteriology·C PissavinN Hugouvieux-Cotte-Pattat
Jun 1, 1993·Applied and Environmental Microbiology·S Kelemu, A Collmer
Feb 14, 1993·Gene·H T Saarilahti
May 1, 1987·Journal of Bacteriology·T K KorhonenD C Old
May 1, 1987·Applied and Environmental Microbiology·L Ward, S H de Boer
Oct 1, 1994·Applied and Environmental Microbiology·A NassarM Lemattre
Aug 1, 1988·Journal of Bacteriology·S J TamakiN T Keen
Nov 25, 2011·Enzyme and Microbial Technology·Sebastián TorresMaría Inés Isla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.