PMID: 9431928Feb 12, 1998Paper

Comparison of performance and cost-effectiveness of direct fluorescent-antibody, ligase chain reaction, and PCR assays for verification of chlamydial enzyme immunoassay results for populations with a low to moderate prevalence of Chlamydia trachomatis infection

Journal of Clinical Microbiology
D DeanM McCarthy

Abstract

Many laboratories use a commercial enzyme immunoassay (EIA) with verification testing to diagnose Chlamydia trachomatis infections in an effort to contain costs. This study was designed to compare the performance and cost-effectiveness of direct fluorescent-antibody assay (DFA), commercial PCR, and ligase chain reaction (LCR) for the verification of EIA results. Cervical specimens were screened by EIA. DFA, PCR, and LCR were compared as verification tests for EIA-reactive specimens and negative greyzone (NGZ) specimens at 50% below the cutoff value. These samples were also tested by in-house PCR, which was used in the analysis of verification results. A total of 477 (7%) of 6,571 samples were reactive or within the NGZ. EIA results with verification by DFA testing (EIA/DFA results) agreed with 93% of the true results compared with 97% for EIA/PCR results for one set of 242 samples; there was 97% agreement with true results for EIA/DFA results versus 95% for EIA/LCR results for another set of 235 samples. Ten samples were false positive by LCR. Time and costs were equivalent for EIA with the DFA, PCR, or LCR as the verification test but were two- to threefold greater for PCR or LCR alone than for EIA with verification. Since it ...Continue Reading

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Citations

Jan 19, 2000·International Journal of STD & AIDS·R S Morton, G R Kinghorn
May 2, 2008·Sexually Transmitted Diseases·Charlotte A GaydosJohn Papp
Apr 3, 2003·Sexually Transmitted Diseases·José A VarelaFernando Vázquez
Jan 27, 2004·Sexually Transmitted Diseases·Diane R BlakeThomas C Quinn
Dec 6, 2002·Journal of Medical Microbiology·Emma J WatsonBabil Stray Pederson
Oct 27, 2006·Journal of Clinical Microbiology·Sue SkidmoreUNKNOWN Chlamydia Screening Studies (ClaSS) Project Group
Mar 27, 2003·American Journal of Preventive Medicine·Katerina Hollblad-Fadiman, Samuel M Goldman
Jul 3, 2002·Infectious Disease Clinics of North America·Gorm LisbyDavid T Durack

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