Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3+ CD5dim CD21- Cytotoxic Large Granular Lymphocytes

Frontiers in Immunology
Soo-Hyeon LeeGuk-Hyun Suh

Abstract

Natural killer (NK) cells play a pivotal role in the immune response against infections and malignant transformation, and adopted transfer of NK cells is thought to be a promising therapeutic approach for cancer patients. Previous reports describing the phenotypic features of canine NK cells have produced inconsistent results. Canine NK cells are still defined as non-B and non-T (CD3-CD21-) large granular lymphocytes. However, a few reports have demonstrated that canine NK cells share the phenotypic characteristics of T lymphocytes, and that CD3+CD5dimCD21- lymphocytes are putative canine NK cells. Based on our previous reports, we hypothesized that phenotypic modulation could occur between these two populations during activation. In this study, we investigated the phenotypic and functional differences between CD3+CD5dimCD21- (cytotoxic large granular lymphocytes) and CD3-CD5-CD21- NK lymphocytes before and after culture of peripheral blood mononuclear cells isolated from normal dogs. The results of this study show that CD3+CD5dimCD21- lymphocytes can be differentiated into non-B, non-T NK (CD3-CD5-CD21-TCRαβ-TCRγδ-GranzymeB+) lymphocytes through phenotypic modulation in response to cytokine stimulation. In vitro studies of pur...Continue Reading

References

May 1, 1985·Veterinary Immunology and Immunopathology·S S Ringler, S Krakowka
Apr 1, 1995·Veterinary Immunology and Immunopathology·Y NakadaF Koide
Feb 10, 1998·Clinical and Diagnostic Laboratory Immunology·R K KatialM M Lieberman
May 1, 2002·European Journal of Immunology·Lorenzo MorettaAlessandro Moretta
May 10, 2006·The Journal of Experimental Medicine·Bertrand MeresseBana Jabri
Apr 18, 2007·Nature Reviews. Immunology·Hans-Gustaf Ljunggren, Karl-Johan Malmberg
May 12, 2007·European Journal of Immunology·Charles A StewartImmo Prinz
May 19, 2007·American Journal of Clinical Pathology·William G Morice
Apr 22, 2008·Nature Immunology·Eric VivierSophie Ugolini
Jul 25, 2008·Blood·Michael A Caligiuri
Aug 19, 2008·Journal of Leukocyte Biology·Yi-Chun HuangRea-Min Chu
Apr 23, 2009·Cancer Research·Hiroyuki FujisakiDario Campana
Jun 16, 2009·Veterinary Immunology and Immunopathology·Elizabeth M GrahamJayne C Hope
Jul 1, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanzhong LiuGary A Visner
Aug 28, 2009·Veterinary Immunology and Immunopathology·Yi-Chun LinRea-Min Chu
Aug 13, 2010·Cancer Immunology, Immunotherapy : CII·Eleni G IliopoulouSonia A Perez
Jul 6, 2011·Tissue Antigens·M InngjerdingenJ T Vaage
Jun 6, 2012·Methods in Molecular Biology·David H McKennaJeffrey S Miller
Nov 10, 2012·The Journal of Reproduction and Development·Yurika TachibanaKazuhiko Imakawa
Mar 5, 2013·Veterinary Research·Kerstin H MairWilhelm Gerner
Apr 4, 2013·Veterinary Immunology and Immunopathology·Dong-Jun ShinSang-Ki Kim
Jul 24, 2013·Veterinary Immunology and Immunopathology·Helen T MichaelJaime F Modiano
Jan 15, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Muriel MalaiséAlexander Kroemer
Feb 20, 2014·Seminars in Immunology·Dorothy K SojkaWayne M Yokoyama
Mar 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timothy K ConnelleyW Ivan Morrison
Mar 29, 2014·Frontiers in Immunology·Salem ChouaibJan Spanholtz
May 27, 2014·Frontiers in Immunology·James J KnoxLaura M McLane
Dec 2, 2014·Veterinary Research Communications·Christine Grøndahl-RosadoAnne K Storset
Feb 15, 2015·Veterinary Microbiology·Ji-Yun ParkSang-Ki Kim
Oct 7, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico SimonettaEddy Roosnek
Jul 6, 2016·Frontiers in Immunology·Federico SimonettaEddy Roosnek
Jul 21, 2016·Veterinary Immunology and Immunopathology·Christine Grøndahl-RosadoAnne K Storset
Mar 1, 2017·Veterinary Immunology and Immunopathology·Kathrin RotheHeiner von Buttlar

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Citations

Nov 14, 2019·Journal of Clinical Medicine·Alicia A GingrichRobert J Canter
Jun 26, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Stanislav PantelyushinJohannes Vom Berg
Feb 6, 2020·Frontiers in Immunology·Steven Dow
Dec 12, 2019·Frontiers in Immunology·Friederike V RabigerGottfried Alber
Mar 7, 2021·Cancers·Stanislav PantelyushinJohannes Vom Berg
Jun 10, 2021·Cytokine·Soo-Hyeon LeeSang-Ki Kim
Aug 24, 2021·Frontiers in Immunology·Hans Klingemann
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Urszula LisieckaStanisław Winiarczyk
Dec 21, 2021·Frontiers in Veterinary Science·Ellen E SpargerMichael S Kent

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Methods Mentioned

BETA
blood draws
density gradient centrifugation
fluorescence-activated cell sorting
flow cytometry
enzyme-linked immunosorbent assay

Software Mentioned

FlowJo
SPSS

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