Comparison of Phenotypic and Genotypic Methods Used for the Species Identification of Lactobacillus NP51 and Development of a Strain-Specific PCR Assay

Probiotics and Antimicrobial Proteins
S RandhawaE Karunasena

Abstract

The species level identity of Lactobacillus NP51, a commercial direct-fed microbial previously identified as Lactobacillus acidophilus NP51, was re-evaluated to determine whether new technologies resulted in changes in the original identification. The phenotypic methods for species identification included API 50 CHL kit and two automated systems, Vitek 2 and MIDI (FAME analysis; a total of three independent FAME analyses). Discrepancies among the identification results with all methods of phenotypic analysis were reported. MicroSeqID 500 16S rRNA system (SeqWright Inc., Houston, TX), a genotypic method, identified the organism as Lactobacillus animalis. Cloning, sequencing and subsequent sequence comparison of NP51 16S-23S intergenic spacer region (ISRs) to nucleotide sequence databases using the BLAST search tool indicated that NP51 can now be named L. animalis. When NP51 was originally identified as L. acidophilus, the designation of L. animalis did not exist taxonomically. The NP51 sequence comparisons using BLAST also revealed that NP51 and a strain previously identified as L. animalis LA51 HOFG1 by Flint and Angert are identical strains under different names. A strain-specific primer pair was also identified for HOFG1 by t...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1994·International Journal of Food Microbiology·R GilarováJ Dobiás
Aug 1, 1996·Current Microbiology·M I CastellanosC Barreau
Dec 1, 1996·Applied and Environmental Microbiology·A L McCartneyG W Tannock
Apr 29, 1997·International Journal of Food Microbiology·M E Stiles, W H Holzapfel
Aug 15, 1998·International Journal of Food Microbiology·G KleinG Reuter
Dec 3, 1998·Applied and Environmental Microbiology·A Tilsala-Timisjärvi, T Alatossava
Jul 31, 1999·Applied and Environmental Microbiology·F E DewhirstJ G Fox
Nov 24, 1999·Current Microbiology·D RoyF Mondou
Feb 7, 2001·The American Journal of Clinical Nutrition·W H HolzapfelU Schillinger
Jun 28, 2002·Journal of Dairy Science·P S M YeungP S Tong
Jun 5, 2003·International Journal of Food Microbiology·Kirsi Brandt, Tapani Alatossava
Feb 22, 2005·Journal of Microbiological Methods·Joseph F Flint, Esther R Angert
Jul 15, 2005·International Journal of Food Microbiology·Isabel SánchezLlanos Palop
Sep 8, 2005·Journal of Clinical Microbiology·Frédéric WalletRené J Courcol
May 4, 2007·Journal of Industrial Microbiology & Biotechnology·Daniela Santos PontesAndréa Maria Amaral Nascimento
Aug 6, 2008·Systematic and Applied Microbiology·Anette McLeodLars Axelsson
Jun 1, 2007·Indian Journal of Microbiology·O PrakashR Lal

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Citations

Jan 22, 2013·Microbial Pathogenesis·Matthieu Million, Didier Raoult
Mar 20, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Behrooz RazbanDunling Wang

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