Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment.

Applied and Environmental Microbiology
V Boivin-JahnsR Christen

Abstract

Seventy-four bacterial strains were freshly isolated from a mine gallery. Using these bacteria, we have investigated how a molecular identification based on the analysis of small subunit rDNA sequences would compare in terms of precision and reliability to a more classical comparison of phenotypical descriptions (100 morphological and physiological traits). Our data clearly showed that a phylogenetic analysis of small subunit rDNA sequences is more efficient than classical phenotypic methods for the identification of bacterial strains freshly isolated from a natural environment, because occurrences of misidentification are very much decreased by this method. The lack of rDNA sequences for many described species is probably the major cause of a few failures in molecular identification, as the completeness of the database of small subunit rDNA sequences holds much importance in the degree of uncertainty in such identifications.

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Aug 14, 1998·Environmental Health Perspectives·T FordJ Shine
Mar 27, 2007·FEMS Microbiology Ecology·Marta NedelkovaSonja Selenska-Pobell
Feb 7, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G KronvallE Falsen
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Apr 1, 1996·Applied and Environmental Microbiology·M G Hofle, I Brettar
Sep 1, 1996·Applied and Environmental Microbiology·V Boivin-JahnsR Christen

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