PMID: 6111322Mar 1, 1981Paper

Comparison of phosphohydrolase activities from articular cartilage in calcium pyrophosphate deposition disease and primary osteoarthritis

Arthritis and Rheumatism
J TenenbaumD S Howell

Abstract

One abnormality in calcium pyrophosphate deposition disease (CPDD) which fosters consistently high synovial fluid pyrophosphate ion (PPi) and large accumulations of calcium pyrophosphate dihydrate crystals (Ca pyrophosphate) might be an aberration in chondrocytes involving elaboration of PPi and failure of its hydrolysis within cartilage matrix. Exploration of this hypothesis required further information on the phosphohydrolases in relevant human articular cartilages. Triton X-100 extracts of whole homogenized cartilage from 18 patients with primary osteoarthritis (OA), 10 patients with CPDD and secondary OA, as well as 6 "normal" subjects were partially purified by DE-52 chromatography and eluates studied for phosphohydrolase activity in a variety of substrates, inhibitors, and environmental conditions. Almost all the protein as well as crude alkaline phosphatase and pyrophosphatase activities were clustered in peaks designated I and II. Findings in CPDD cartilage not observed in OA controls were: 1) consistent alkaline phosphatase activity in the void volume of DE-52 columns, 2) high levels of 5'nucleotidase activity, 3) abundant generation of PPi by CPDD cartilage during in vitro incubation of cartilage extract fractions wit...Continue Reading

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Citations

Jan 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B O OyajobiR G Russell
Sep 1, 1987·Calcified Tissue International·G K HunterK P Pritzker
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Oct 15, 2003·Arthritis and Rheumatism·Maryse PicherGreta M Lee
Jan 1, 1985·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T A EinhornR P Carty
Dec 1, 1985·Arthritis and Rheumatism·J W Rachow, L M Ryan
Dec 1, 1986·Arthritis and Rheumatism·A P PrinsJ K van der Korst
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Aug 1, 1989·Archives of Biochemistry and Biophysics·A K RosenthalL M Ryan

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