PMID: 6169848Oct 1, 1981Paper

Comparison of phosphorylation of two polyoma virus middle T antigens in vivo and in vitro

Journal of Virology
B Schaffhausen, T L Benjamin

Abstract

Two species of polyoma virus middle T antigen were detected in both lytically infected and transformed cells by in vitro kinase assay of immunoprecipitates. A minor species with an apparent molecular weight of 58,000 (58K) represented less than 10% of the total middle T protein. This species was roughly 10 times more active as a phosphate acceptor than was the predominant 56K form. Partial proteolytic mapping experiments showed that the same site was phosphorylated in both species. Mapping of the middle T antigens from a series of deletion mutants suggested that the major site of phosphorylation is tyrosine residue 315. Phosphorylation occurred on both middle T species in vivo, involving sites predominantly other than the tyrosine labeled in vitro. The 56K and 58K middle T forms differed from each other in their in vivo phosphorylation patterns. Some phosphate was incorporated into the 58K species in a region of the molecule to which at least part of the apparent molecular weight different could be mapped. hr-t mutant NG-59, which codes for a slightly altered middle T, produced only a single species (56K) which was inactive in the in vitro kinase reaction. Moreover, no 58K species appeared in vivo with this mutant. hr-t mutants...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·E G Krebs, J A Beavo
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y ItoR Dulbecco
Aug 1, 1975·Virology·E Goldman, T L Benjamin
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B S Schaffhausen, T L Benjamin
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J FeunteunT Benjamin
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B S SchaffhausenT L Benjamin
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M S Collett, R L Erikson
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T HunterW Eckhart
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R E KaressH Hanafusa
Nov 1, 1979·Journal of Virology·G Magnusson, P Berg
Sep 1, 1979·Journal of Virology·B E Griffin, C Maddock
Dec 1, 1979·Cell·B S Schaffhausen, T L Benjamin
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·T L Benjamin
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Jan 1, 1980·Cold Spring Harbor Symposia on Quantitative Biology·R KamenA Mellor
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S KawaiK Toyoshima
Feb 1, 1963·Virology·E WINOCOUR

❮ Previous
Next ❯

Citations

Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Michele M Fluck, Brian S Schaffhausen
Jan 1, 1982·CRC Critical Reviews in Biochemistry·B Schaffhausen
Feb 3, 1986·European Journal of Biochemistry·K Palme, W Eckhart
May 26, 1995·The Journal of Biological Chemistry·W SuT M Roberts
Nov 20, 1998·Molecular and Cellular Biology·K P MullaneB Schaffhausen
Jan 1, 1986·Cancer Investigation·D S Salomon, I Perroteau
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·K A Gottlieb, L P Villarreal
Nov 18, 2016·Journal of Virology·Deborah DenisBrian S Schaffhausen
Jul 28, 1995·Biochimica Et Biophysica Acta·B Schaffhausen
Jun 1, 1990·Journal of Virology·L St-OngeM Bastin
Apr 1, 1986·Journal of Virology·J B BolenC Prives
May 1, 1990·Journal of Virology·D N Cook, J A Hassell
Feb 1, 1994·Journal of Virology·P S HolmanB S Schaffhausen
Apr 1, 1987·Journal of Virology·B J Bockus, B Schaffhausen
Apr 1, 1987·Journal of Virology·B S SchaffhausenT M Roberts
Jan 1, 1984·Pharmacology & Therapeutics·C Basilico
Nov 25, 1987·Biochimica Et Biophysica Acta·W Markland, A E Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.