Comparison of polymerase chain reaction-restriction enzyme analysis method and DNA sequence analysis results in the identification of non-tuberculous mycobacteria

Acta Microbiologica Et Immunologica Hungarica
Özgür AppakAyşe Aydan Özkütük

Abstract

The typing of non-tuberculous mycobacteria (NTM) is important from a clinical and epidemiological perspective. The polymerase chain reaction-restriction enzyme analysis (PRA) method and DNA sequence analysis method were utilized to target a gene region that codes the 65-kDa heat-shock protein for typing 150 suspected NTM samples isolated from the respiratory tract. Mycobacterium abscessus, Mycobacterium xenopi, Mycobacterium fortuitum, and Mycobacterium peregrinum were most frequently found by both methods. Six isolates that could not be defined by the PRA method were defined as Nocardia cyriacigeorgica, Nocardia abscessus, and Mycobacterium intracellulare by DNA sequence analysis. Discordance between the results of the two methods was observed for only one isolate. The isolate that was defined as Mycobacterium gordonae type 6 by the PRA method was defined as Mycobacterium senegalense by sequence analysis. The PRA method is simple and gives rapid results. Compared with DNA sequence analysis, it gives consistent and reliable results up to a ratio of 90%. DNA sequence analysis is the gold standard method in which all strains can be defined. However, given our laboratory conditions, its disadvantage is that it takes longer to reac...Continue Reading

References

Feb 13, 1999·Journal of Clinical Microbiology·H RinguetC Pierre-Audigier
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Debby V CousinsAmelia Bernardelli
Jul 15, 2005·International Journal of Systematic and Evolutionary Microbiology·Hong KimBum-Joon Kim
Nov 26, 2005·BMC Infectious Diseases·Won Sup OhNam Yong Lee
Feb 6, 2007·American Journal of Respiratory and Critical Care Medicine·David E GriffithUNKNOWN Infectious Disease Society of America
Oct 1, 2009·Emerging Infectious Diseases·Claudio Piersimoni, Claudio Scarparo
Nov 13, 2009·Journal of Clinical Microbiology·Enrico TortoliAnna Fabio
May 5, 2012·Tüberküloz ve toraks·Aylin BabalıkKaya Köksalan
Sep 4, 2012·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Rachel TalavlikarSusan Kuhn
Nov 23, 2013·Annals of Clinical Microbiology and Antimicrobials·Murat GunaydinRiza Durmaz
Dec 15, 2015·International Journal of Mycobacteriology·Ali Akbar VelayatiMehdi Mirsaeidi

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Citations

May 18, 2019·Acta Microbiologica Et Immunologica Hungarica·Francesca LosaStefano R Del Giacco

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