Comparison of polymerase chain reaction with culture and serology for diagnosis of murine experimental Lyme borreliosis.

Journal of Clinical Microbiology
A R PachnerE Delaney

Abstract

After the intradermal inoculation of mice with Borrelia burgdorferi, the antibody response, culture, and histology of blood and target organs were assessed and compared with results of a nested polymerase chain reaction (PCR) assay. Of 247 specimens of heart, brain, bladder, and blood, the tested concordance between the PCR and culture was 72%. In the 69 instances of discordance, the PCR was positive in 51 and the culture was positive in 18; thus, the PCR was concordant or more sensitive in 93% of the tested organs. In mice infected with 10 spirochetes, serology confirmed by Western blotting (immunoblotting) was more sensitive than either culture or PCR of brain, bladder, or heart specimens. The organs most commonly culture or PCR positive were the heart and bladder; the brain was infected in only 26% of the animals. DNA hybridization was helpful in confirming the PCR product as being specific and, in some cases, in demonstrating a positive product in the face of negative agarose gels. PCR was less sensitive than culture in detecting the presence of spirochetes in blood specimens, possibly because of the presence of blood inhibitors. We thus found a nested PCR assay, using primers from a genomic sequence, to be a valuable adjun...Continue Reading

References

Jan 1, 1991·Journal of Clinical Microbiology·J M BurstainJ D Radolf
Mar 1, 1991·Journal of Clinical Microbiology·P A RosaT G Schwan
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·G ZimmerM M Simon
Nov 22, 1990·The New England Journal of Medicine·E L LogigianA C Steere
Jun 1, 1990·The Journal of Infectious Diseases·J C Garcia-MoncoJ L Benach
May 1, 1989·Trends in Neurosciences·A R Pachner
Feb 1, 1989·Seminars in Arthritis and Rheumatism·L H Sigal
Aug 31, 1989·The New England Journal of Medicine·A C Steere
Jan 1, 1988·Annals of the New York Academy of Sciences·P H Duray, A C Steere
Apr 1, 1988·The Journal of Infectious Diseases·S W BartholdA C Steere
Jan 1, 1988·Annals of the New York Academy of Sciences·S W BartholdA C Steere
Dec 1, 1984·Journal of Clinical Microbiology·R C JohnsonC Kodner

❮ Previous
Next ❯

Citations

Oct 1, 1993·Annals of Neurology·A R Pachner, E Delaney
Sep 7, 2004·Annals of Neurology·Andrew R PachnerDiego Cadavid
Jan 1, 1996·Springer Seminars in Immunopathology·A R Pachner
Nov 1, 1996·Antimicrobial Agents and Chemotherapy·R J KazragisA G Barbour

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.