Comparison of primary human gingival fibroblasts from an older and a young donor on the evaluation of cytotoxicity of denture adhesives

Journal of Applied Oral Science : Revista FOB
Aline S L Santoro SoaresGutemberg Alves

Abstract

Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6). Gingival fibroblasts isolated from a 30- and a 62-year-old donor were assayed for proliferation (1-7 days) and sensitivity to latex (positive control). Fibroblasts were indirectly exposed to Corega Ultra (cream), Corega powder and Fixodent Original for a 24 h period and assayed by XTT and Crystal Violet tests. The release of IL-6 and bFGF by exposed cells was determined by ELISA. While cells from the young donor presented higher cell growth after 7 days, the sensitivity to increasing concentrations of latex extracts was very similar between young and older cells. Bo...Continue Reading

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Citations

Jul 14, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Sergio López-GarcíaFrancisco J Rodríguez-Lozano
Aug 1, 2020·Odontology·Andréa Vaz Braga PintorGutemberg Gomes Alves
Dec 18, 2020·Brazilian Oral Research·Larissa Maria CavalcanteLuis Felipe Jochims Schneider
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Yuan LiuHui Cheng
Apr 24, 2021·The Journal of Prosthetic Dentistry·Rayanna Thayse Florêncio CostaSandra Lúcia Dantas Moraes

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
ELISA

Software Mentioned

GraphPad
GraphPad Prism

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