Comparison of primer sets for T-cell clonality testing in canine intestinal lymphoma

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Masamine Takanosu, Yumiko Kagawa

Abstract

Clonality testing based on polymerase chain reaction is an important tool for diagnosis of lymphoproliferative diseases. Many primers have been designed and used for canine clonality testing. Canine intestinal lymphoma is usually diagnosed pathologically by examination of excised intestinal or endoscopic biopsy tissues, and clonality testing is sometimes used to support the pathological diagnosis if this examination is inconclusive. In the present study, the sensitivity of each previously published primer set for clonality testing was examined by using formalin-fixed, paraffin-embedded sections from 39 cases pathologically diagnosed as canine intestinal lymphoma (large-cell type). All 39 cases were immunohistochemically positive for cluster of differentiation (CD)3. Thirty-two out of the 39 cases showed clonality in the T-cell receptor gamma (TRG) with at least 1 of the tested primers. The primer set with the highest sensitivity detected all 32 cases with TRG clonality, with a sensitivity of 82.1%. These results provide useful evidence for the selection of primer sets for clonality testing of canine intestinal lymphoma.

References

Mar 16, 2004·Veterinary Pathology·K A Coyle, H Steinberg
Mar 6, 2007·The Veterinary Clinics of North America. Small Animal Practice·Anne C Avery, Paul R Avery
Aug 12, 2009·Journal of Hematopathology·Patricia J T A GroenenJohan H J M van Krieken
Sep 10, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gabrita De ZanMassimo Castagnaro
Jan 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Pascal ChaubertMonika Welle
Jan 11, 2012·Veterinary Immunology and Immunopathology·Stefan M Keller, Peter F Moore
Jan 13, 2012·Veterinary Immunology and Immunopathology·Stefan M Keller, Peter F Moore
Jan 31, 2012·The Veterinary Clinics of North America. Small Animal Practice·Anne C Avery
Dec 3, 2013·The Journal of Veterinary Medical Science·Yuko NakanoMasamine Takanosu

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Citations

Oct 27, 2016·The Journal of Small Animal Practice·J R S Dandrieux

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Methods Mentioned

BETA
biopsy
PCR
PCRs
electrophoresis

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