Abstract
In order to select a better prostate cancer cell model for androgen receptor (AR)-mediated reporter gene assays, we assessed the androgen response characteristics of three cell lines, LNCaP, PC3/AR(+) and 22Rv1, in this study. Both the mRNA and the proteins of AR and glucocorticoid receptor (GR) were expressed in all three cell lines. Among the three cell lines, only in LNCaP cells, DHT concentration-dependently stimulated proliferation. DHT induced the luciferase activity in three cell lines which were transiently transfected with pMMTV-Luc, in a concentration-dependent manner. The maximum induction was 24.0-fold and 13.4-fold in 22Rv1 and in the LNCaP respectively. PC3/AR(+) were more sensitive to respond to DHT at a minimal concentration of 10(-12)M by 14.0-fold induction. The transcriptional activity induced with 10(-8)M DHT was inhibited about 50-75% in the PC3/AR(+) and 22Rv1, and 98% in the LNCaP, by vinclozolin. Dexamethasone concentration-dependently induced the luciferase activity in PC3 and 22Rv1, but not in the LNCaP. However, the response to dexamethasone in 22Rv1 was very weak compared to DHT. The (anti)androgencity of seven pyrethroids was assessed via an AR-mediated luciferase reporter assay. None of them showed...Continue Reading
References
Mar 15, 1978·International Journal of Cancer. Journal International Du Cancer·K R StoneD F Paulson
Dec 14, 1990·Biochemical and Biophysical Research Communications·J VeldscholteE Mulder
Aug 1, 1986·Molecular and Cellular Biology·P DarbreR J King
Aug 25, 1995·The Journal of Biological Chemistry·C WongE M Wilson
Mar 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·M BorrásG Leclercq
Sep 4, 1998·Reproduction, Fertility, and Development·W R KelceE M Wilson
Oct 29, 1998·Biochemical and Biophysical Research Communications·J Garey, M S Wolff
Dec 16, 1998·Cellular and Molecular Life Sciences : CMLS·B J Danzo
Aug 26, 1999·In Vitro Cellular & Developmental Biology. Animal·R M SramkoskiJ W Jacobberger
Oct 26, 1999·International Journal of Urology : Official Journal of the Japanese Urological Association·T TakahashiS Orikasa
Jan 5, 2000·The Prostate·P J GkonosK L Burnstein
May 2, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·C J WolfL E Gray
Feb 27, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·D M Tessier, F Matsumura
Feb 28, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Vickie S WilsonL E Gray
Feb 28, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·P C HartigL E Gray
Mar 9, 2002·Toxicology and Applied Pharmacology·Helle Raun AndersenEva Cecilie Bonefeld-Jørgensen
Mar 26, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hyun Ju LeeKeesook Lee
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·A HartelHeinrich H D Meyer
May 15, 2003·The Prostate·Samuel R DenmeadeJohn T Isaacs
Dec 31, 2003·Mutation Research·Il-Hyun KangMi-Sook Dong
Jan 23, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cynthia J WolfL Earl Gray
Jul 14, 2004·Molecular and Cellular Endocrinology·Barbara J AttardiJerry R Reel
Nov 3, 2004·Toxicology and Applied Pharmacology·Mia BirkhøjAnne Marie Vinggaard
Dec 16, 2004·The Journal of Reproduction and Development·Soon-Sun KimKui-Lea Park
Citations
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chen-Lin HsiehPhilip W Kantoff
Dec 2, 2014·Journal of Toxicology·Gargi Bagchi Bhattacharjee, S M Paul Khurana
Feb 14, 2013·PloS One·Ryan F OvercashMaria J Ruiz-Echevarría
Aug 26, 2010·Applied Microbiology and Biotechnology·Katerina Svobodová, Tomás Cajthaml
Feb 13, 2016·Reproductive Toxicology·Seunghan SunMi-Sook Dong
Nov 20, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Carmela CampanaWilliam E Rainey
May 2, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Andrea DovioAlberto Angeli
Jul 1, 2008·Reproductive Toxicology·Jun ZhangXinqiang Zhu
Nov 6, 2007·Toxicology·Li-Chun XuJi-Hong Shao
Feb 28, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anne-Marie SaillenfaitJean-Philippe Sabaté
Nov 1, 2007·International Journal of Andrology·Bart van der BurgEdwin Sonneveld
May 5, 2011·The Prostate·Sigal Samuel-MendelsohnRonnie J Barkey
Jun 20, 2014·Journal of Ethnopharmacology·Claudia BobachLudger A Wessjohann
Dec 3, 2014·Toxicology Letters·Ivana KlopčičMarija Sollner Dolenc
Apr 16, 2020·BMC Cancer·Yoshinori MatsudaTomonori Habuchi
Jun 21, 2020·Cells·Hadjer DellalPhilippe Pourquier
Jan 8, 2021·Investigational New Drugs·Ahmed ElgehamaQiang Xu
Feb 9, 2019·Environmental Research·Hee-Seok LeeYooheon Park
Feb 9, 2018·Chemosphere·Hee-Seok LeeYong Eui Koo
Aug 30, 2008·Environmental Science & Technology·Sander C Van der LindenBart Van der Burg
Dec 25, 2021·The Prostate·Karolina SienkiewiczAakrosh Ratan