Comparison of prostate cancer cell lines for androgen receptor-mediated reporter gene assays

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Hyun-Jung KimMi-Sook Dong

Abstract

In order to select a better prostate cancer cell model for androgen receptor (AR)-mediated reporter gene assays, we assessed the androgen response characteristics of three cell lines, LNCaP, PC3/AR(+) and 22Rv1, in this study. Both the mRNA and the proteins of AR and glucocorticoid receptor (GR) were expressed in all three cell lines. Among the three cell lines, only in LNCaP cells, DHT concentration-dependently stimulated proliferation. DHT induced the luciferase activity in three cell lines which were transiently transfected with pMMTV-Luc, in a concentration-dependent manner. The maximum induction was 24.0-fold and 13.4-fold in 22Rv1 and in the LNCaP respectively. PC3/AR(+) were more sensitive to respond to DHT at a minimal concentration of 10(-12)M by 14.0-fold induction. The transcriptional activity induced with 10(-8)M DHT was inhibited about 50-75% in the PC3/AR(+) and 22Rv1, and 98% in the LNCaP, by vinclozolin. Dexamethasone concentration-dependently induced the luciferase activity in PC3 and 22Rv1, but not in the LNCaP. However, the response to dexamethasone in 22Rv1 was very weak compared to DHT. The (anti)androgencity of seven pyrethroids was assessed via an AR-mediated luciferase reporter assay. None of them showed...Continue Reading

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Citations

Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chen-Lin HsiehPhilip W Kantoff
Dec 2, 2014·Journal of Toxicology·Gargi Bagchi Bhattacharjee, S M Paul Khurana
Aug 26, 2010·Applied Microbiology and Biotechnology·Katerina Svobodová, Tomás Cajthaml
Nov 20, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Carmela CampanaWilliam E Rainey
Jul 1, 2008·Reproductive Toxicology·Jun ZhangXinqiang Zhu
Feb 28, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anne-Marie SaillenfaitJean-Philippe Sabaté
Nov 1, 2007·International Journal of Andrology·Bart van der BurgEdwin Sonneveld
May 5, 2011·The Prostate·Sigal Samuel-MendelsohnRonnie J Barkey
Aug 30, 2008·Environmental Science & Technology·Sander C Van der LindenBart Van der Burg

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