PMID: 7334378Dec 1, 1981Paper

Comparison of proteoglycans extracted by saline and guanidinium chloride from cultured chick retinas

Journal of Neurochemistry
J E Morris, Y P Ting

Abstract

To compare the loosely associated sulfated proteoglycans with those tightly bound to membranes, retinas from 14-day chick embryos were subjected to progressively disruptive techniques. The most easily removed proteoglycans were isolated from the medium in which the tissue was labeled with [35S]sulfate. On the average, 25% of the glycosaminoglycans were in the labeling medium, 39% were in proteoglycans extracted from the tissue in the balanced salt solution, 32% were in a 4 M-guanidinium chloride (GuCl) fraction, and 4% remained unextracted. These glycosaminoglycans contained, respectively, 28, 28, 40, and 4% of the incorporated [35S]sulfate. On the basis of electrophoretic mobility and TLC of chondroitinase digests, the ratio of 35S in chondroitin sulfate to that in heparan sulfate was 4-7 times higher in the medium and balanced salt extracts than in the GuCl extracts. In both extracts there was more 35S in chondroitin-6-sulfate than in chondroitin-4-sulfate. Dialysis of the extracts against 0.5 M-NaCl resulted in the precipitation of about 12% of the glycosaminoglycans in the saline extracts and about 40% in GuCl extract. These subfractions, which were relatively enriched in heparan sulfate, were largely soluble in dithiothrei...Continue Reading

References

Apr 19, 1976·Biochemical and Biophysical Research Communications·J E Morris, A Dorfman
Aug 1, 1976·The Biochemical Journal·P J Roughley, R M Mason
Jan 1, 1977·Biochemical Society Transactions·C J White
Sep 1, 1979·Histochemistry·M MareelG Dacremont
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R K MargolisR U Margolis
Apr 1, 1979·The Journal of Cell Biology·K HedmanM Höök
Mar 1, 1977·Journal of Neurochemistry·C J White, M Hudson
Feb 1, 1979·Analytical Biochemistry·L Hronowski, T P Anastassiades
May 1, 1978·Journal of Neurochemistry·R K MargolisR U Margolis
Aug 15, 1978·Experientia·C J White
Jul 15, 1977·Developmental Biology·J E MorrisA Dorfman
May 1, 1973·Developmental Biology·L Feeney
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M J Palmoski, P F Goetinck
Dec 21, 1971·Biochimica Et Biophysica Acta·G Bach, E R Berman
Dec 21, 1971·Biochimica Et Biophysica Acta·G Bach, E R Berman
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Dec 20, 1976·Biochemical and Biophysical Research Communications·R U MargolisR K Margolis

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Citations

Apr 1, 1989·Developmental Biology·A ThrelkeldA T Hewitt
Sep 5, 2002·Progress in Retinal and Eye Research·Masaru Inatani, Hidenobu Tanihara
Jul 22, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nikoletta NagyMárta Széll
Oct 9, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dana M CairnsLi Zeng
Mar 1, 1984·Journal of Neurochemistry·J E MorrisA Birkholz-Lambrecht

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